Mechanical-induced bone remodeling does not depend on Piezo1 in dentoalveolar hard tissue.

Autor: Nottmeier C; Department of Orthodontics, University of Leipzig Medical Center, Saxony, Germany., Lavicky J; Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic., Gonzalez Lopez M; Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic., Knauth S; Department of Orthodontics, University of Leipzig Medical Center, Saxony, Germany., Kahl-Nieke B; Department of Orthodontics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Amling M; Institute of Osteology and Biomechanics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Schinke T; Institute of Osteology and Biomechanics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Helms J; Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford University, Palo Alto, CA, USA., Krivanek J; Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic., Koehne T; Department of Orthodontics, University of Leipzig Medical Center, Saxony, Germany. till.koehne@medizin.uni-leipzig.de., Petersen J; Department of Orthodontics, University of Leipzig Medical Center, Saxony, Germany. julian.petersen@medizin.uni-leipzig.de.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Jun 12; Vol. 13 (1), pp. 9563. Date of Electronic Publication: 2023 Jun 12.
DOI: 10.1038/s41598-023-36699-9
Abstrakt: Mechanosensory ion channels are proteins that are sensitive to mechanical forces. They are found in tissues throughout the body and play an important role in bone remodeling by sensing changes in mechanical stress and transmitting signals to bone-forming cells. Orthodontic tooth movement (OTM) is a prime example of mechanically induced bone remodeling. However, the cell-specific role of the ion channels Piezo1 and Piezo2 in OTM has not been investigated yet. Here we first identify the expression of PIEZO1/2 in the dentoalveolar hard tissues. Results showed that PIEZO1 was expressed in odontoblasts, osteoblasts, and osteocytes, while PIEZO2 was localized in odontoblasts and cementoblasts. We therefore used a Piezo1 floxed/floxed mouse model in combination with Dmp1 cre to inactivate Piezo1 in mature osteoblasts/cementoblasts, osteocytes/cementocytes, and odontoblasts. Inactivation of Piezo1 in these cells did not affect the overall morphology of the skull but caused significant bone loss in the craniofacial skeleton. Histological analysis revealed a significantly increased number of osteoclasts in Piezo1 floxed/floxed ;Dmp1 cre mice, while osteoblasts were not affected. Despite this increased number of osteoclasts, orthodontic tooth movement was not altered in these mice. Our results suggest that despite Piezo1 being crucial for osteoclast function, it may be dispensable for mechanical sensing of bone remodeling.
(© 2023. The Author(s).)
Databáze: MEDLINE
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