Memory phase-specific genes in the Mushroom Bodies identified using CrebB-target DamID.

Autor: Sgammeglia N; Department of Biology, University of Fribourg, Fribourg, Switzerland., Widmer YF; Department of Biology, University of Fribourg, Fribourg, Switzerland., Kaldun JC; Department of Biology, University of Fribourg, Fribourg, Switzerland., Fritsch C; Department of Biology, University of Fribourg, Fribourg, Switzerland., Bruggmann R; Interfaculty Bioinformatics Unit and Swiss Institute of Bioinformatics, University of Bern, Bern, Switzerland., Sprecher SG; Department of Biology, University of Fribourg, Fribourg, Switzerland.
Jazyk: angličtina
Zdroj: PLoS genetics [PLoS Genet] 2023 Jun 12; Vol. 19 (6), pp. e1010802. Date of Electronic Publication: 2023 Jun 12 (Print Publication: 2023).
DOI: 10.1371/journal.pgen.1010802
Abstrakt: The formation of long-term memories requires changes in the transcriptional program and de novo protein synthesis. One of the critical regulators for long-term memory (LTM) formation and maintenance is the transcription factor CREB. Genetic studies have dissected the requirement of CREB activity within memory circuits, however less is known about the genetic mechanisms acting downstream of CREB and how they may contribute defining LTM phases. To better understand the downstream mechanisms, we here used a targeted DamID approach (TaDa). We generated a CREB-Dam fusion protein using the fruit fly Drosophila melanogaster as model. Expressing CREB-Dam in the mushroom bodies (MBs), a brain center implicated in olfactory memory formation, we identified genes that are differentially expressed between paired and unpaired appetitive training paradigm. Of those genes we selected candidates for an RNAi screen in which we identified genes causing increased or decreased LTM.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Sgammeglia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje