Biophysical and Biochemical Regulation of Cell Dynamics in Magnetically Assembled Cellular Structures.

Autor:	Gupta T; School of Biomedical Engineering, McMaster University, Hamilton, Ontario L8S 4L8, Canada., Sahu RP; School of Biomedical Engineering, McMaster University, Hamilton, Ontario L8S 4L8, Canada.; Department of Mechanical Engineering, McMaster University, Hamilton, Ontario L8S 4L8, Canada.; Department of Materials Science and Engineering, McMaster University, Hamilton, Ontario L8S 4L8, Canada., Dabaghi M; Firestone Institute for Respiratory Health-Division of Respirology, Dept of Medicine, McMaster University, Hamilton, Ontario L8S 4L8, Canada., Zhong LS; Integrated Biomedical Engineering & Health Sciences, McMaster University, Hamilton, Ontario L8S 4K1, Canada., Shargall Y; Division of Thoracic Surgery, Department of Surgery, McMaster University, St. Joseph's Healthcare Hamilton, 50 Charlton Avenue East, Hamilton, Ontario L8N 4A6, Canada., Hirota JA; School of Biomedical Engineering, McMaster University, Hamilton, Ontario L8S 4L8, Canada.; Firestone Institute for Respiratory Health-Division of Respirology, Dept of Medicine, McMaster University, Hamilton, Ontario L8S 4L8, Canada., Richards CD; McMaster Immunology Research Centre, Department of Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada., Puri IK; School of Biomedical Engineering, McMaster University, Hamilton, Ontario L8S 4L8, Canada.; Department of Aerospace and Mechanical Engineering, University of Southern California, Los Angeles, California 90089, United States.; Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, California 90089, United States.
Jazyk:	angličtina
Zdroj:	ACS omega [ACS Omega] 2023 May 25; Vol. 8 (22), pp. 19976-19986. Date of Electronic Publication: 2023 May 25 (Print Publication: 2023).
DOI:	10.1021/acsomega.3c02052
Abstrakt:	Soluble signaling molecules and extracellular matrix (ECM) regulate cell dynamics in various biological processes. Wound healing assays are widely used to study cell dynamics in response to physiological stimuli. However, traditional scratch-based assays can damage the underlying ECM-coated substrates. Here, we use a rapid, non-destructive, label-free magnetic exclusion technique to form annular aggregates of bronchial epithelial cells on tissue-culture treated (TCT) and ECM-coated surfaces within 3 h. The cell-free areas enclosed by the annular aggregates are measured at different times to assess cell dynamics. The effects of various signaling molecules, including epidermal growth factor (EGF), oncostatin M, and interleukin 6, on cell-free area closures are investigated for each surface condition. Surface characterization techniques are used to measure the topography and wettability of the surfaces. Further, we demonstrate the formation of annular aggregates on human lung fibroblast-laden collagen hydrogel surfaces, which mimic the native tissue architecture. The cell-free area closures on hydrogels indicate that the substrate properties modulate EGF-mediated cell dynamics. The magnetic exclusion-based assay is a rapid and versatile alternative to traditional wound healing assays. Competing Interests: The authors declare no competing financial interest. (© 2023 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
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