Comparative performance evaluation of blood film microscopy for the diagnosis of bovine trypanosomosis by some laboratories in North-central Nigeria.

Autor: Akinbobola JS; Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Abuja, Abuja, Nigeria., Okaiyeto SO; Veterinary Teaching Hospital, Ahmadu Bello University, Zaria, Nigeria., Sackey AK; Department of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria., Allam L; Veterinary Teaching Hospital, Ahmadu Bello University, Zaria, Nigeria., Mohammed B; Department of Veterinary Pathology, Ahmadu Bello University, Zaria, Nigeria., Dinga JN; Michael Gahnyam Gbeugvat Foundation, Buea, Cameroon.; Biotechnology Unit, Faculty of Science, University of Buea, Buea, Cameroon., Chukwuemeka PC; Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Abuja, Abuja, Nigeria., Cunningham L; Department of Tropical Disease Biology, Biological Sciences, Liverpool School of Tropical Medicine, Liverpool, UK.
Jazyk: angličtina
Zdroj: Open veterinary journal [Open Vet J] 2023 May; Vol. 13 (5), pp. 599-603. Date of Electronic Publication: 2023 May 13.
DOI: 10.5455/OVJ.2023.v13.i5.12
Abstrakt: Background: Due to its affordability in disease-affected communities and suitability for field application, microscopy has historically been considered the gold standard for field diagnosis of trypanosomosis in rural settings.
Aim: This works aims to compare the performance of microscopists on bovine trypanosome microscopy by organizing the first comparative assessment on a correct reading of slides by laboratory professionals using the read slide results and a structured interviewer-administered questionnaire in North-central Nigeria.
Methods: Ten participants were addressed, as they were sent a panel of two slides (Slide 1: No Trypanosome present; Slide 2: Trypanosome present) and a questionnaire.
Results: All participants greater than 41 years old reported correctly the presence and absence of parasites on slides. Only 3/8 of microscopists from routine diagnostic laboratories reported correctly the presence of the parasite.
Conclusion: Our study confirmed errors in reading slides. Therefore, training of microscopists besides a nationwide quality assessment is recommended.
Competing Interests: The authors declare that there is no conflict of interest regarding the publication of this paper.
Databáze: MEDLINE