Sex-dependent differences in behavioral and immunological responses to antibiotic and bacteriophage administration in mice.

Autor:	Grabowski Ł; Laboratory of Bacteriophage Therapy, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Gdansk, Poland., Pierzynowska K; Department of Molecular Biology, Faculty of Biology, University of Gdansk, Gdansk, Poland., Kosznik-Kwaśnicka K; Laboratory of Bacteriophage Therapy, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Gdansk, Poland.; Department of Medical Microbiology, Faculty of Medicine, Medical University of Gdansk, Gdansk, Poland., Stasiłojć M; Department of Molecular Biology, Faculty of Biology, University of Gdansk, Gdansk, Poland.; Department of Cell Biology and Immunology, Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdansk, Gdansk, Poland., Jerzemowska G; Department of Animal and Human Physiology, Faculty of Biology, University of Gdansk, Gdansk, Poland., Węgrzyn A; Phage Therapy Center, University Center of Applied and Interdisciplinary Research, Gdansk, Poland., Węgrzyn G; Department of Molecular Biology, Faculty of Biology, University of Gdansk, Gdansk, Poland., Podlacha M; Department of Molecular Biology, Faculty of Biology, University of Gdansk, Gdansk, Poland.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2023 May 25; Vol. 14, pp. 1133358. Date of Electronic Publication: 2023 May 25 (Print Publication: 2023).
DOI:	10.3389/fimmu.2023.1133358
Abstrakt:	Introduction: The problem of antibiotic resistance is a global one, involving many industries and entailing huge financial outlays. Therefore, the search for alternative methods to combat drug-resistant bacteria has a priority status. Great potential is seen in bacteriophages which have the natural ability to kill bacterial cells. Bacteriophages also have several advantages over antibiotics. Firstly, they are considered ecologically safe (harmless to humans, plants and animals). Secondly, bacteriophages preparations are readily producible and easy to apply. However, before bacteriophages can be authorized for medical and veterinary use, they must be accurately characterized in vitro and in vivo to determinate safety. Methods: Therefore, the aim of this study was to verify for the first time the behavioral and immunological responses of both male and female mice (C57BL/6J) to bacteriophage cocktail, composed of two bacteriophages, and to two commonly used antibiotics, enrofloxacin and tetracycline. Animal behavior, the percentage of lymphocyte populations and subpopulations, cytokine concentrations, blood hematological parameters, gastrointestinal microbiome analysis and the size of internal organs, were evaluated. Results: Unexpectedly, we observed a sex-dependent, negative effect of antibiotic therapy, which not only involved the functioning of the immune system, but could also significantly impaired the activity of the central nervous system, as manifested by disruption of the behavioral pattern, especially exacerbated in females. In contrast to antibiotics, complex behavioral and immunological analyses confirmed the lack of adverse effects during the bacteriophage cocktail administration. Discussion: The mechanism of the differences between males and females in appearance of adverse effects, related to the behavioral and immune functions, in the response to antibiotic treatment remains to be elucidated. One might imagine that differences in hormones and/or different permeability of the blood-brain barrier can be important factors, however, extensive studies are required to find the real reason(s). Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Grabowski, Pierzynowska, Kosznik-Kwaśnicka, Stasiłojć, Jerzemowska, Węgrzyn, Węgrzyn and Podlacha.)
Databáze:	MEDLINE
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