The effects of ambient temperature exposure on feline fecal metabolome.

Autor:	Chiu O; Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada., Tal M; Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada., Sanmugam A; Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada., Hesta M; Department of Morphology, Imaging, Orthopedics, Rehabilitation and Nutrition, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium., Gomez DE; Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada., Weese JS; Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada., Verbrugghe A; Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2023 May 26; Vol. 10, pp. 1141881. Date of Electronic Publication: 2023 May 26 (Print Publication: 2023).
DOI:	10.3389/fvets.2023.1141881
Abstrakt:	Introduction: The fecal metabolome provides insight into overall gastrointestinal and microbial health. Methods for fecal sample storage in metabolomics research vary, however, making comparisons within current literature difficult. This study investigated the effect of ambient temperature exposure on microbial-derived metabolites of feline fecal samples. Methods: Fecal samples were collected from 11 healthy cats from a local boarding facility. Samples were manually homogenized and aliquoted. The first aliquot was frozen at -80°C within 1 hour of defecation, and remaining samples were exposed to ambient temperature for 2, 4, 6, 8, 12, and 24 h prior to freezing at -80°C. Fecal metabolites were quantified using 1 H NMR spectroscopy. Fifty metabolites were grouped into six categories (27 amino acids, 8 fatty acids, 5 sugars, 3 alcohols, 2 nitrogenous bases, 5 miscellaneous). Results: Concentrations of 20 out of 50 metabolites significantly differed due to ambient temperature exposure (7 amino acids, 6 fatty acids, 2 alcohols, 1 nitrogenous base, 4 miscellaneous). The earliest detected changes occurred 6 h post-defecation for cadaverine and fumaric acid. Discussion: This study shows ambient temperature exposure alters the composition of the feline fecal metabolome, but short-term (up to 4 h) exposure prior to storage in the freezer seems to be acceptable. Competing Interests: AV is the Royal Canin Veterinary Diet Endowed Chair in Canine and Feline Clinical Nutrition at the Ontario Veterinary College. MT is currently employed by Royal Canin. The study was conducted before this employment as part of MT’s Doctor Veterinary Sciences Degree and OC’s Master of Sciences Degree at the Ontario Veterinary College. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Chiu, Tal, Sanmugam, Hesta, Gomez, Weese and Verbrugghe.)
Databáze:	MEDLINE
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