Investigating parasites in three dimensions: trends in volume microscopy.

Autor: Cooper C; Centre for Microscopy, Characterisation, and Analysis, University of Western Australia, Stirling Hwy, Crawley, WA 6009, Australia. Electronic address: crystal.cooper@uwa.edu.au., Thompson RCA; School of Veterinary and Life Sciences, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia., Clode PL; Centre for Microscopy, Characterisation, and Analysis, University of Western Australia, Stirling Hwy, Crawley, WA 6009, Australia; School of Biological Sciences, University of Western Australia, Stirling Hwy, Crawley, WA 6009, Australia.
Jazyk: angličtina
Zdroj: Trends in parasitology [Trends Parasitol] 2023 Aug; Vol. 39 (8), pp. 668-681. Date of Electronic Publication: 2023 Jun 09.
DOI: 10.1016/j.pt.2023.05.004
Abstrakt: To best understand parasite, host, and vector morphologies, host-parasite interactions, and to develop new drug and vaccine targets, structural data should, ideally, be obtained and visualised in three dimensions (3D). Recently, there has been a significant uptake of available 3D volume microscopy techniques that allow collection of data across centimetre (cm) to Angstrom (Å) scales by utilising light, X-ray, electron, and ion sources. Here, we present and discuss microscopy tools available for the collection of 3D structural data, focussing on electron microscopy-based techniques. We highlight their strengths and limitations, such that parasitologists can identify techniques best suited to answer their research questions. Additionally, we review the importance of volume microscopy to the advancement of the field of parasitology.
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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