Optimization of human skin keratinocyte culture protocols using bioactive molecules derived from olive oil.

Autor: Ortiz-Arrabal O; Tissue Engineering Group, Department of Histology, Faculty of Medicine, University of Granada, Granada E18016, Spain; Instituto de Investigación Biosanitaria ibs.GRANADA, Granada E18012, Spain; Doctoral Program in Biochemistry and Molecular Biology, University of Granada, Granada E18071, Spain., Bermejo-Casares F; Tissue Engineering Group, Department of Histology, Faculty of Medicine, University of Granada, Granada E18016, Spain., Garzón I; Tissue Engineering Group, Department of Histology, Faculty of Medicine, University of Granada, Granada E18016, Spain; Instituto de Investigación Biosanitaria ibs.GRANADA, Granada E18012, Spain., Mesa-García MD; Instituto de Investigación Biosanitaria ibs.GRANADA, Granada E18012, Spain; Department of Biochemistry and Molecular Biology II, School of Pharmacy, Campus Universitario de Cartuja, Granada 18071, Spain; Institute of Nutrition and Food Technology 'José Mataix', Biomedical Research Center, University of Granada, Avenida del Conocimiento s/n, Granada 18100, Spain. Electronic address: mdmesa@ugr.es., Gómez-Llorente C; Instituto de Investigación Biosanitaria ibs.GRANADA, Granada E18012, Spain; Department of Biochemistry and Molecular Biology II, School of Pharmacy, Campus Universitario de Cartuja, Granada 18071, Spain; Institute of Nutrition and Food Technology 'José Mataix', Biomedical Research Center, University of Granada, Avenida del Conocimiento s/n, Granada 18100, Spain. Electronic address: gomezll@ugr.es., Alaminos M; Tissue Engineering Group, Department of Histology, Faculty of Medicine, University of Granada, Granada E18016, Spain; Instituto de Investigación Biosanitaria ibs.GRANADA, Granada E18012, Spain. Electronic address: malaminos@ugr.es.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2023 Aug; Vol. 164, pp. 115000. Date of Electronic Publication: 2023 Jun 08.
DOI: 10.1016/j.biopha.2023.115000
Abstrakt: Skin damage due to severe burns can compromise patient life. Current tissue engineering methods allow the generation of human skin substitutes for clinical use. However, this process is time-consuming, as the keratinocytes required to generate artificial skin have a low proliferation rate in culture. In this study, we evaluated the pro-proliferative effects of three natural biomolecules isolated from olive oil: phenolic extract (PE), DL-3,4-dihydroxyphenyl glycol (DHFG), and oleuropein (OLP), on cultured human skin keratinocytes. The results showed that PE and OLP increased the proliferation of immortalized human skin keratinocytes, especially at concentrations of 10 and 5 µg/mL, respectively, without altering cell viability. In contrast, DHFG did not produce a significant improvement in keratinocyte proliferation. In normal human skin keratinocytes obtained from skin biopsies, we found that PE, but not OLP, could increase the number of keratinocyte colonies and the area occupied by these cells. Furthermore, this effect was associated with increased KI-67 and Proliferating cell nuclear antigen (PCNA) gene expression. Thus, we propose that PE positively affects keratinocyte proliferation and could be used in culture protocols to improve bioartificial skin generation by tissue engineering.
Competing Interests: Declaration of Competing Interest Authors declare that they do not have any conflicts of interest.
(Copyright © 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: