| Autor: |
Montesissa M; Department of Biomedical and Neuromotor Sciences, University of Bologna, 40138 Bologna, Italy., Borciani G; Department of Biomedical and Neuromotor Sciences, University of Bologna, 40138 Bologna, Italy., Rubini K; Department of Chemistry 'Giacomo Ciamician', University of Bologna, 40126 Bologna, Italy., Valle F; Institute of Nanostructured Materials, National Research Council, 40129 Bologna, Italy., Boi M; BST Biomedical Science and Technologies and Nanobiotechnology Laboratory, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy., Baldini N; Department of Biomedical and Neuromotor Sciences, University of Bologna, 40138 Bologna, Italy.; BST Biomedical Science and Technologies and Nanobiotechnology Laboratory, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy., Boanini E; Department of Chemistry 'Giacomo Ciamician', University of Bologna, 40126 Bologna, Italy., Graziani G; BST Biomedical Science and Technologies and Nanobiotechnology Laboratory, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy. |
| Abstrakt: |
Calcium phosphate-based coatings are widely studied in orthopedics and dentistry for their similarity to the mineral component of bone and their capability to promote osseointegration. Different calcium phosphates have tunable properties that result in different behaviors in vitro, but the majority of studies focus only on hydroxyapatite. Here, different calcium phosphate-based nanostructured coatings are obtained by ionized jet deposition, starting with hydroxyapatite, brushite and beta-tricalcium phosphate targets. The properties of the coatings obtained from different precursors are systematically compared by assessing their composition, morphology, physical and mechanical properties, dissolution, and in vitro behavior. In addition, for the first time, depositions at high temperature are investigated for the further tuning of the coatings mechanical properties and stability. Results show that different phosphates can be deposited with good composition fidelity even if not in a crystalline phase. All coatings are nanostructured and non-cytotoxic and display variable surface roughness and wettability. Upon heating, higher adhesion and hydrophilicity are obtained as well as higher stability, resulting in better cell viability. Interestingly, different phosphates show very different in vitro behavior, with brushite being the most suitable for promoting cell viability and beta-tricalcium phosphate having a higher impact on cell morphology at the early timepoints. |
