| Autor: |
Rabchinskii MK; Ioffe Institute, Politekhnicheskaya St. 26, Saint Petersburg 194021, Russia., Besedina NA; Department of Physics, Alferov University, 8/3 Khlopina Street, Saint Petersburg 194021, Russia., Brzhezinskaya M; Helmholtz-Zentrum Berlin für Materialien und Energie, Hahn-Meitner-Platz 1, 14109 Berlin, Germany., Stolyarova DY; NRC 'Kurchatov Institute', Akademika Kurchatova pl. 1, Moscow 123182, Russia., Ryzhkov SA; Ioffe Institute, Politekhnicheskaya St. 26, Saint Petersburg 194021, Russia., Saveliev SD; Ioffe Institute, Politekhnicheskaya St. 26, Saint Petersburg 194021, Russia., Antonov GA; Ioffe Institute, Politekhnicheskaya St. 26, Saint Petersburg 194021, Russia., Baidakova MV; Ioffe Institute, Politekhnicheskaya St. 26, Saint Petersburg 194021, Russia., Pavlov SI; Ioffe Institute, Politekhnicheskaya St. 26, Saint Petersburg 194021, Russia., Kirilenko DA; Ioffe Institute, Politekhnicheskaya St. 26, Saint Petersburg 194021, Russia., Shvidchenko AV; Ioffe Institute, Politekhnicheskaya St. 26, Saint Petersburg 194021, Russia., Cherviakova PD; Ioffe Institute, Politekhnicheskaya St. 26, Saint Petersburg 194021, Russia., Brunkov PN; Ioffe Institute, Politekhnicheskaya St. 26, Saint Petersburg 194021, Russia. |
| Abstrakt: |
The facile synthesis of biografted 2 D derivatives complemented by a nuanced understanding of their properties are keystones for advancements in biosensing technologies. Herein, we thoroughly examine the feasibility of aminated graphene as a platform for the covalent conjugation of monoclonal antibodies towards human IgG immunoglobulins. Applying core-level spectroscopy methods, namely X-ray photoelectron and absorption spectroscopies, we delve into the chemistry and its effect on the electronic structure of the aminated graphene prior to and after the immobilization of monoclonal antibodies. Furthermore, the alterations in the morphology of the graphene layers upon the applied derivatization protocols are assessed by electron microscopy techniques. Chemiresistive biosensors composed of the aerosol-deposited layers of the aminated graphene with the conjugated antibodies are fabricated and tested, demonstrating a selective response towards IgM immunoglobulins with a limit of detection as low as 10 pg/mL. Taken together, these findings advance and outline graphene derivatives' application in biosensing as well as hint at the features of the alterations of graphene morphology and physics upon its functionalization and further covalent grafting by biomolecules. |
