| Autor: |
Samaniego-Gámez BY; Institute of Agricultural Sciences, Autonomous University of Baja California, Delta Highway s/n Ejido Nuevo León, Mexicali P.O. Box 21705, Baja California, Mexico., Valle-Gough RE; Institute of Agricultural Sciences, Autonomous University of Baja California, Delta Highway s/n Ejido Nuevo León, Mexicali P.O. Box 21705, Baja California, Mexico., Garruña-Hernández R; CONACYT-National Technological Institute of Mexico, Technological Institute of Conkal, CONACYT, Tecnológico Ave. s/n, Conkal P.O. Box 97345, Yucatán, Mexico., Reyes-Ramírez A; National Technological Institute of Mexico, Conkal Institute of Technology, Division of Graduate Studies and Research, Av. Tecnológico s/n, Conkal P.O. Box 97345, Yucatán, Mexico., Latournerie-Moreno L; National Technological Institute of Mexico, Conkal Institute of Technology, Division of Graduate Studies and Research, Av. Tecnológico s/n, Conkal P.O. Box 97345, Yucatán, Mexico., Tun-Suárez JM; National Technological Institute of Mexico, Conkal Institute of Technology, Division of Graduate Studies and Research, Av. Tecnológico s/n, Conkal P.O. Box 97345, Yucatán, Mexico., Villanueva-Alonzo HJ; Regional Research Center 'Dr. Hideyo Noguchi', Cell Biology Laboratory, Autonomous University of Yucatan, Av. Itzáez, Nmbr. 490 by 59 St. Centro, Merida P.O. Box 97000, Yucatán, Mexico., Nuñez-Ramírez F; Institute of Agricultural Sciences, Autonomous University of Baja California, Delta Highway s/n Ejido Nuevo León, Mexicali P.O. Box 21705, Baja California, Mexico., Diaz LC; Institute of Agricultural Sciences, Autonomous University of Baja California, Delta Highway s/n Ejido Nuevo León, Mexicali P.O. Box 21705, Baja California, Mexico., Samaniego-Gámez SU; Institute of Agricultural Sciences, Autonomous University of Baja California, Delta Highway s/n Ejido Nuevo León, Mexicali P.O. Box 21705, Baja California, Mexico., Minero-García Y; Yucatan Center of Scientific Research, Plant Biochemistry and Molecular Biology Unit, 43 St., Nmbr. 130, Chuburna de Hidalgo, Merida P.O. Box 97200, Yucatán, Mexico., Hernandez-Zepeda C; Yucatan Center of Scientific Research, Water Sciences Unit, 8 St., Nmbr. 39, SM 64, Mz. 29, Cancun P.O. Box 77500, Quintana Roo, Mexico., Moreno-Valenzuela OA; Yucatan Center of Scientific Research, Plant Biochemistry and Molecular Biology Unit, 43 St., Nmbr. 130, Chuburna de Hidalgo, Merida P.O. Box 97200, Yucatán, Mexico. |
| Abstrakt: |
Induced systemic resistance (ISR) is a mechanism involved in the plant defense response against pathogens. Certain members of the Bacillus genus are able to promote the ISR by maintaining a healthy photosynthetic apparatus, which prepares the plant for future stress situations. The goal of the present study was to analyze the effect of the inoculation of Bacillus on the expression of genes involved in plant responses to pathogens, as a part of the ISR, during the interaction of Capsicum chinense infected with PepGMV. The effects of the inoculation of the Bacillus strains in pepper plants infected with PepGMV were evaluated by observing the accumulation of viral DNA and the visible symptoms of pepper plants during a time-course experiment in greenhouse and in in vitro experiments. The relative expression of the defense genes CcNPR1 , CcPR10 , and CcCOI1 were also evaluated. The results showed that the plants inoculated with Bacillus subtilis K47, Bacillus cereus K46, and Bacillus sp. M9 had a reduction in the PepGMV viral titer, and the symptoms in these plants were less severe compared to the plants infected with PepGMV and non-inoculated with Bacillus . Additionally, an increase in the transcript levels of CcNPR1 , CcPR10 , and CcCOI1 was observed in plants inoculated with Bacillus strains. Our results suggest that the inoculation of Bacillus strains interferes with the viral replication, through the increase in the transcription of pathogenesis-related genes, which is reflected in a lowered plant symptomatology and an improved yield in the greenhouse, regardless of PepGMV infection status. |
