| Autor: |
Menolli RA; Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel 85819-110, PR, Brazil.; Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80240-020, PR, Brazil., Tessaro FHG; Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo 05508-000, SP, Brazil., do Amaral AE; Unidade de Laboratório de Análises Clínicas, Universidade Federal de Santa Catarina, Florianópolis 88036-800, SC, Brazil., de Melo RH; Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel 85819-110, PR, Brazil., Dos Santos JF; Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80240-020, PR, Brazil., Iacomini M; Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba 81531-980, PR, Brazil., Smiderle FR; Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80240-020, PR, Brazil., Mello RG; Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80240-020, PR, Brazil. |
| Abstrakt: |
C. brachyspora , a widespread dematiaceous fungus, was evaluated in this study to optimize the production of exopolysaccharides (CB-EPS). Optimization was performed using response surface methodology, and the best production yielded 75.05% of total sugar at pH 7.4, with 0.1% urea, after 197 h. The obtained CB-EPS showed typical signals of polysaccharides, which was confirmed by FT-IR and NMR. The HPSEC analysis indicated a polydisperse polymer, showing a non-uniform peak, with an average molar mass (M w ) of 24,470 g/mol. The major monosaccharide was glucose (63.9 Mol%), followed by mannose (19.7 Mol%), and galactose (16.4 Mol%). Methylation analysis encountered derivatives that indicated the presence of a β-d-glucan and a highly branched glucogalactomannan. CB-EPS was tested on murine macrophages to verify its immunoactivity, and the treated cells were able to produce TNF-α, IL-6, and IL-10. However, the cells did not produce superoxide anions or nitric oxide nor stimulated phagocytosis. The results demonstrated an indirect antimicrobial activity of macrophages by stimulating cytokines, showing another biotech applicability for the exopolysaccharides produced by C. brachyspora . |
