Effects of chemical inhibition of histone deacetylase proteins in the growth and virulence of Macrophomina phaseolina (Tassi) Goid.

Autor:	Villota-Salazar NA; Biotecnología Vegetal, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Blvd. del Maestro s/n esq. Elías Piña, Col. Narciso Mendoza, 88710 Reynosa, Tamaulipas, Mexico., Ramos-García VH; Biotecnología Vegetal, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Blvd. del Maestro s/n esq. Elías Piña, Col. Narciso Mendoza, 88710 Reynosa, Tamaulipas, Mexico., González-Prieto JM; Biotecnología Vegetal, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Blvd. del Maestro s/n esq. Elías Piña, Col. Narciso Mendoza, 88710 Reynosa, Tamaulipas, Mexico., Hernández-Delgado S; Biotecnología Vegetal, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Blvd. del Maestro s/n esq. Elías Piña, Col. Narciso Mendoza, 88710 Reynosa, Tamaulipas, Mexico. Electronic address: shernandezd@ipn.mx.
Jazyk:	angličtina
Zdroj:	Revista Argentina de microbiologia [Rev Argent Microbiol] 2023 Oct-Dec; Vol. 55 (4), pp. 296-306. Date of Electronic Publication: 2023 Jun 07.
DOI:	10.1016/j.ram.2023.04.002
Abstrakt:	Chromatin remodeling enzymes are important "writers", "readers" and "erasers" of the epigenetic code. These proteins are responsible for the placement, recognition, and removal of molecular marks in histone tails that trigger structural and functional changes in chromatin. This is also the case for histone deacetylases (HDACs), i.e., enzymes that remove acetyl groups from histone tails, signaling heterochromatin formation. Chromatin remodeling is necessary for cell differentiation processes in eukaryotes, and fungal pathogenesis in plants includes many adaptations to cause disease. Macrophomina phaseolina (Tassi) Goid. is a nonspecific, necrotrophic ascomycete phytopathogen that causes charcoal root disease. M. phaseolina is a frequent and highly destructive pathogen in crops such as common beans (Phaseolus vulgaris L.), particularly under both water and high temperature stresses. Here, we evaluated the effects of the classical HDAC inhibitor trichostatin A (TSA) on M. phaseolinain vitro growth and virulence. During inhibition assays, the growth of M. phaseolina in solid media, as well as the size of the microsclerotia, were reduced (p<0.05), and the colony morphology was remarkably affected. Under greenhouse experiments, treatment with TSA reduced (p<0.05) fungal virulence in common bean cv. BAT 477. Tests of LIPK, MAC1 and PMK1 gene expression during the interaction of fungi with BAT 477 revealed noticeable deregulation. Our results provide additional evidence about the role of HATs and HDACs in important biological processes of M. phaseolina. (Copyright © 2023 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu