Centromere/kinetochore is assembled through CENP-C oligomerization.

Autor:	Hara M; Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan. Electronic address: mahara@fbs.osaka-u.ac.jp., Ariyoshi M; Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan., Sano T; Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan., Nozawa RS; Division of Experimental Pathology, Cancer Institute of the Japanese Foundation for Cancer Research, Tokyo 135-8550, Japan., Shinkai S; Laboratory for Developmental Dynamics, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan., Onami S; Laboratory for Developmental Dynamics, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan., Jansen I; Abberior Instruments GmbH, Göttingen 37077, Germany., Hirota T; Division of Experimental Pathology, Cancer Institute of the Japanese Foundation for Cancer Research, Tokyo 135-8550, Japan., Fukagawa T; Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan. Electronic address: fukagawa.tatsuo.fbs@osaka-u.ac.jp.
Jazyk:	angličtina
Zdroj:	Molecular cell [Mol Cell] 2023 Jul 06; Vol. 83 (13), pp. 2188-2205.e13. Date of Electronic Publication: 2023 Jun 08.
DOI:	10.1016/j.molcel.2023.05.023
Abstrakt:	Kinetochore is an essential protein complex required for accurate chromosome segregation. The constitutive centromere-associated network (CCAN), a subcomplex of the kinetochore, associates with centromeric chromatin and provides a platform for the kinetochore assembly. The CCAN protein CENP-C is thought to be a central hub for the centromere/kinetochore organization. However, the role of CENP-C in CCAN assembly needs to be elucidated. Here, we demonstrate that both the CCAN-binding domain and the C-terminal region that includes the Cupin domain of CENP-C are necessary and sufficient for chicken CENP-C function. Structural and biochemical analyses reveal self-oligomerization of the Cupin domains of chicken and human CENP-C. We find that the CENP-C Cupin domain oligomerization is vital for CENP-C function, centromeric localization of CCAN, and centromeric chromatin organization. These results suggest that CENP-C facilitates the centromere/kinetochore assembly through its oligomerization. Competing Interests: Declaration of interests I.J. is an employee of Abberior Instruments, which develops and manufactures super-resolution fluorescence microscopes, including the MINFLUX system used here. All other authors declare no competing interests. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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