Mitochondrial uncouplers impair human sperm motility without altering ATP content†.

Autor:	Skinner WM; Endocrinology Graduate Group, University of California, Berkeley, Berkeley, California, USA.; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA., Petersen NT; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.; Department of Obstetrics and Gynecology, Stanford University School of Medicine, Palo Alto, California, USA., Unger B; Department of Chemistry, University of California, Berkeley, Berkeley, California, USA., Tang S; Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA.; Sarafan ChEM-H, Stanford University, Stanford, California, USA., Tabarsi E; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.; Keck School of Medicine, University of Southern California, Los Angeles, California, USA., Lamm J; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.; Dewpoint Therapeutics, Boston, Massachusetts, USA., Jalalian L; Department of Obstetrics and Gynecology, University of California, San Francisco Center for Reproductive Health, San Francisco, California, USA., Smith J; Department of Urology, University of California, San Francisco, San Francisco, California, USA., Bertholet AM; Department of Physiology, University of California, San Francisco, San Francisco, California, USA.; Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California, USA., Xu K; Department of Chemistry, University of California, Berkeley, Berkeley, California, USA.; California Institute for Quantitative Biosciences, University of California, Berkeley, California, USA.; Chan Zuckerberg Biohub, San Francisco, California, USA., Kirichok Y; Department of Physiology, University of California, San Francisco, San Francisco, California, USA., Lishko PV; Endocrinology Graduate Group, University of California, Berkeley, Berkeley, California, USA.; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.; Department of Cell Biology & Physiology, Washington University School of Medicine in St. Louis, St. Louis, Missouri, USA.
Jazyk:	angličtina
Zdroj:	Biology of reproduction [Biol Reprod] 2023 Aug 10; Vol. 109 (2), pp. 192-203.
DOI:	10.1093/biolre/ioad064
Abstrakt:	In human spermatozoa, the electrochemical potentials across the mitochondrial and plasma membranes are related to sperm functionality and fertility, but the exact role of each potential has yet to be clarified. Impairing sperm mitochondrial function has been considered as an approach to creating male or unisex contraceptives, but it has yet to be shown whether this approach would ultimately block the ability of sperm to reach or fertilize an egg. To investigate whether the mitochondrial and plasma membrane potentials are necessary for sperm fertility, human sperm were treated with two small-molecule mitochondrial uncouplers (niclosamide ethanolamine and BAM15) that depolarize membranes by inducing passive proton flow, and evaluated the effects on a variety of sperm physiological processes. BAM15 specifically uncoupled human sperm mitochondria while niclosamide ethanolamine induced proton current in the plasma membrane in addition to depolarizing the mitochondria. In addition, both compounds significantly decreased sperm progressive motility with niclosamide ethanolamine having a more robust effect. However, these uncouplers did not reduce sperm adenosine triphosphate (ATP) content or impair other physiological processes, suggesting that human sperm can rely on glycolysis for ATP production if mitochondria are impaired. Thus, systemically delivered contraceptives that target sperm mitochondria to reduce their ATP production would likely need to be paired with sperm-specific glycolysis inhibitors. However, since niclosamide ethanolamine impairs sperm motility through an ATP-independent mechanism, and niclosamide is FDA approved and not absorbed through mucosal membranes, it could be a useful ingredient in on-demand, vaginally applied contraceptives. (© The Author(s) 2023. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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