Protein cohabitation: long-term immunoglobulin G storage at room temperature.

Autor: Bharmoria P; Department of Chemistry and Molecular Biology, University of Gothenburg, Kemigården 10, 412 96, Gothenburg, Sweden. alesia.a.tietze@gu.se.; Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Kemivägen 4, 412 96, Gothenburg, Sweden. kasper.moth-poulsen@chalmers.se.; The Institute of Materials Science of Barcelona, ICMAB-CSIC, Bellaterra, 08193, Barcelona, Spain. pbharmoria@icmab.es., Ooi SA; Department of Chemistry and Molecular Biology, University of Gothenburg, Kemigården 10, 412 96, Gothenburg, Sweden. alesia.a.tietze@gu.se., Cellini A; Department of Chemistry and Molecular Biology, University of Gothenburg, Kemigården 10, 412 96, Gothenburg, Sweden. alesia.a.tietze@gu.se., Tietze D; Department of Chemistry and Molecular Biology, University of Gothenburg, Kemigården 10, 412 96, Gothenburg, Sweden. alesia.a.tietze@gu.se., Maj M; Department of Chemistry - Ångström Laboratory, Physical Chemistry, Lägerhyddsvägen 1, 751 20 Uppsala, Sweden., Moth-Poulsen K; Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Kemivägen 4, 412 96, Gothenburg, Sweden. kasper.moth-poulsen@chalmers.se.; The Institute of Materials Science of Barcelona, ICMAB-CSIC, Bellaterra, 08193, Barcelona, Spain. pbharmoria@icmab.es.; Catalan Institution for Research & Advanced Studies, ICREA, Pg. Lluís Companys 23, Barcelona, Spain.; Department of Chemical Engineering, Universitat Politècnica de Catalunya, EEBE, Eduard Maristany 10-14, 08019 Barcelona, Spain., Tietze AA; Department of Chemistry and Molecular Biology, University of Gothenburg, Kemigården 10, 412 96, Gothenburg, Sweden. alesia.a.tietze@gu.se.
Jazyk: angličtina
Zdroj: Journal of materials chemistry. B [J Mater Chem B] 2023 Jun 21; Vol. 11 (24), pp. 5400-5405. Date of Electronic Publication: 2023 Jun 21.
DOI: 10.1039/d3tb00161j
Abstrakt: Long-term functional storage of therapeutic proteins at room temperature has been an eternal challenge. Inspired by the cellular cooperativity of proteins, we have taken a step forward to address this challenge by cohabitating Immunoglobulin G (IgG1) with a food protein gelatin in the solid-state at room temperature. Interestingly, IgG1 remained functionally active for a record 14 months revealed from the western-blot assay. Further quantification by HP-LC analysis showed 100% structural integrity of IgG1 with no degradation in the gelatin matrix during this period. The developed formulation has a direct application in oral medical nutrition therapy to cure gastrointestinal microbial infections. Also the strategy provides a robust energy economic alternative to the protein engineering methods for long-term functional storage of therapeutic proteins at room temperature.
Databáze: MEDLINE