Normoglycemia partially recovers the disrupted osteoblast differentiation of mesenchymal stem cells induced by type 1 but not type 2 diabetes mellitus.

Autor: Weffort D; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil., Adolpho LF; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil., Souza ATP; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil., Freitas GP; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.; School of Dentistry, Federal University of Goiás, Goiânia, Brazil., Lopes HB; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil., Oliveira FS; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil., Bighetti-Trevisan RL; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil., Pitol-Palin L; Department of Basic Sciences, Araçatuba Dental School, São Paulo State University Júlio de Mesquita Filho, Araçatuba, Brazil., Matsushita DH; Department of Basic Sciences, Araçatuba Dental School, São Paulo State University Júlio de Mesquita Filho, Araçatuba, Brazil., Okamoto R; Department of Basic Sciences, Araçatuba Dental School, São Paulo State University Júlio de Mesquita Filho, Araçatuba, Brazil., Beloti MM; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil., Rosa AL; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
Jazyk: angličtina
Zdroj: Journal of cellular biochemistry [J Cell Biochem] 2023 Jul; Vol. 124 (7), pp. 1050-1063. Date of Electronic Publication: 2023 Jun 09.
DOI: 10.1002/jcb.30434
Abstrakt: Type 1 (T1DM) and type 2 (T2DM) diabetes mellitus are characterized by changes in glucose metabolism and cause bone damage via a variety of mechanisms, including effects on osteoblasts. We aimed to evaluate the osteoblast differentiation of mesenchymal stem cells (MSCs) from rats with T1DM or T2DM and the effects of removing the hyperglycemic stimulus on the osteogenic potential of these cells. MSCs from healthy rats were cultured in normoglycemic medium, whereas MSCs from rats with T1DM or T2DM were cultured in hyperglycemic or normoglycemic medium. T1DM and T2DM reduced osteoblast differentiation of MSCs grown in hyperglycemic media, with T1DM having a more pronounced effect, as evidenced by alkaline phosphatase activity, RUNX2 protein expression, and extracellular matrix mineralization, and modulated the gene expression of several components of the bone morphogenetic protein signaling pathway. The restoration of the normoglycemic environment partially recovers the osteogenic potential of MSCs from rats with T1DM but not with T2DM. Our findings highlight the need for specific therapies to treat T1DM- or T2DM-induced bone loss, as both disrupt osteoblast differentiation at distinct levels and likely through different mechanisms.
(© 2023 Wiley Periodicals LLC.)
Databáze: MEDLINE
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