3D-bioprinting of patient-derived cardiac tissue models for studying congenital heart disease.

Autor:	Wolfe JT; Department of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United States., He W; Department of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United States., Kim MS; Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, United States., Liang HL; Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, United States., Shradhanjali A; Department of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United States., Jurkiewicz H; Department of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United States., Freudinger BP; Engineering Core, Medical College of Wisconsin, Milwaukee, WI, United States., Greene AS; The Jackson Laboratory, Bar Harbor, ME, United States., LaDisa JF Jr; Department of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United States.; Department of Pediatrics - Section of Cardiology, Children's Wisconsin, Milwaukee, WI, United States.; The Herma Heart Institute, Children's Wisconsin, Milwaukee, WI, United States.; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States., Tayebi L; School of Dentistry, Marquette University, Milwaukee, WI, United States., Mitchell ME; Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, United States.; The Herma Heart Institute, Children's Wisconsin, Milwaukee, WI, United States., Tomita-Mitchell A; Department of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United States.; Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, United States.; The Herma Heart Institute, Children's Wisconsin, Milwaukee, WI, United States.; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States., Tefft BJ; Department of Biomedical Engineering, Medical College of Wisconsin & Marquette University, Milwaukee, WI, United States.; Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in cardiovascular medicine [Front Cardiovasc Med] 2023 May 24; Vol. 10, pp. 1162731. Date of Electronic Publication: 2023 May 24 (Print Publication: 2023).
DOI:	10.3389/fcvm.2023.1162731
Abstrakt:	Introduction: Congenital heart disease is the leading cause of death related to birth defects and affects 1 out of every 100 live births. Induced pluripotent stem cell technology has allowed for patient-derived cardiomyocytes to be studied in vitro. An approach to bioengineer these cells into a physiologically accurate cardiac tissue model is needed in order to study the disease and evaluate potential treatment strategies. Methods: To accomplish this, we have developed a protocol to 3D-bioprint cardiac tissue constructs comprised of patient-derived cardiomyocytes within a hydrogel bioink based on laminin-521. Results: Cardiomyocytes remained viable and demonstrated appropriate phenotype and function including spontaneous contraction. Contraction remained consistent during 30 days of culture based on displacement measurements. Furthermore, tissue constructs demonstrated progressive maturation based on sarcomere structure and gene expression analysis. Gene expression analysis also revealed enhanced maturation in 3D constructs compared to 2D cell culture. Discussion: This combination of patient-derived cardiomyocytes and 3D-bioprinting represents a promising platform for studying congenital heart disease and evaluating individualized treatment strategies. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (© 2023 Wolfe, He, Kim, Liang, Shradhanjali, Jurkiewicz, Freudinger, Greene, LaDisa, Tayebi, Mitchell, Tomita-Mitchell and Tefft.)
Databáze:	MEDLINE
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