Coupling and uncoupling of midline morphogenesis and cell flow in amniote gastrulation.

Autor:	Asai R; Cardiovascular Research Institute, University of California, San Francisco. San Francisco, California 94158, USA., Prakash VN; Department of Physics, University of Miami, Coral Gables, Florida 33146, USA., Sinha S; Department of Physics, University of Miami, Coral Gables, Florida 33146, USA., Prakash M; Department of Bioengineering, Stanford University, Stanford, California 94305, USA., Mikawa T; Cardiovascular Research Institute, University of California, San Francisco. San Francisco, California 94158, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Feb 01. Date of Electronic Publication: 2024 Feb 01.
DOI:	10.1101/2023.05.26.542486
Abstrakt:	Large-scale cell flow characterizes gastrulation in animal development. In amniote gastrulation, particularly in avian gastrula, a bilateral vortex-like counter-rotating cell flow, called 'polonaise movements', appears along the midline. Here, through experimental manipulations, we addressed relationships between the polonaise movements and morphogenesis of the primitive streak, the earliest midline structure in amniotes. Suppression of the Wnt/planar cell polarity (PCP) signaling pathway maintains the polonaise movements along a deformed primitive streak. Mitotic arrest leads to diminished extension and development of the primitive streak and maintains the early phase of the polonaise movements. Ectopically induced Vg1, an axis-inducing morphogen, generates the polonaise movements, aligned to the induced midline, but disturbs the stereotypical cell flow pattern at the authentic midline. Despite the altered cell flow, induction and extension of the primitive streak are preserved along both authentic and induced midlines. Finally, we show that ectopic axis-inducing morphogen, Vg1, is capable of initiating the polonaise movements without concomitant PS extension under mitotic arrest conditions. These results are consistent with a model wherein primitive streak morphogenesis is required for the maintenance of the polonaise movements, but the polonaise movements are not necessarily responsible for primitive streak morphogenesis. Our data describe a previously undefined relationship between the large-scale cell flow and midline morphogenesis in gastrulation. Competing Interests: Competing interests: Competing interests No competing interests declared.
Databáze:	MEDLINE
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