Distinct T cell functional profiles in SARS-CoV-2 seropositive and seronegative children associated with endemic human coronavirus cross-reactivity.

Autor:	Benede NSB; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Division of Medical Virology, Department of Pathology, University of Cape Town, Observatory, South Africa., Tincho MB; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Division of Medical Virology, Department of Pathology, University of Cape Town, Observatory, South Africa., Walters A; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Division of Medical Virology, Department of Pathology, University of Cape Town, Observatory, South Africa., Subbiah V; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Division of Medical Virology, Department of Pathology, University of Cape Town, Observatory, South Africa., Ngomti A; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Division of Medical Virology, Department of Pathology, University of Cape Town, Observatory, South Africa., Baguma R; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Division of Medical Virology, Department of Pathology, University of Cape Town, Observatory, South Africa., Butters C; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa., Mennen M; Department of Medicine, University of Cape Town and Groote Schuur Hospital, Observatory, South Africa., Skelem S; Department of Medicine, University of Cape Town and Groote Schuur Hospital, Observatory, South Africa., Adriaanse M; Department of Medicine, University of Cape Town and Groote Schuur Hospital, Observatory, South Africa., van Graan S; National Institute for Communicable Diseases of the National Health Laboratory Services, Johannesburg, South Africa.; MRC Antibody Immunity Research Unit, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa., Balla SR; National Institute for Communicable Diseases of the National Health Laboratory Services, Johannesburg, South Africa.; MRC Antibody Immunity Research Unit, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa., Moyo-Gwete T; National Institute for Communicable Diseases of the National Health Laboratory Services, Johannesburg, South Africa.; MRC Antibody Immunity Research Unit, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa., Moore PL; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; National Institute for Communicable Diseases of the National Health Laboratory Services, Johannesburg, South Africa.; MRC Antibody Immunity Research Unit, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa.; Centre for the AIDS Programme of Research in South Africa, Durban, South Africa., Botha M; Department of Paediatrics and Child Health, University of Cape Town, Cape Town, South Africa.; Medical Research Council (MRC) Unit on Child and Adolescent Health, University of Cape Town, Cape Town, South Africa., Workman L; Department of Paediatrics and Child Health, University of Cape Town, Cape Town, South Africa.; Medical Research Council (MRC) Unit on Child and Adolescent Health, University of Cape Town, Cape Town, South Africa., Zar HJ; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Department of Paediatrics and Child Health, University of Cape Town, Cape Town, South Africa.; Medical Research Council (MRC) Unit on Child and Adolescent Health, University of Cape Town, Cape Town, South Africa.; Wellcome Centre for Infectious Diseases Research in Africa, University of Cape Town, Observatory, South Africa., Ntusi NAB; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Department of Medicine, University of Cape Town and Groote Schuur Hospital, Observatory, South Africa.; Wellcome Centre for Infectious Diseases Research in Africa, University of Cape Town, Observatory, South Africa.; Cape Heart Institute, Faculty of Health Sciences, University of Cape Town, Observatory, South Africa., Zühlke L; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Department of Paediatrics and Child Health, University of Cape Town, Cape Town, South Africa.; Cape Heart Institute, Faculty of Health Sciences, University of Cape Town, Observatory, South Africa.; South African Medical Research Council, Francie Van Zijl Drive, Parow Cape Town, South Africa., Webb K; South African Medical Research Council, Francie Van Zijl Drive, Parow Cape Town, South Africa.; Crick African Network, The Francis Crick Institute, London, United Kingdom., Riou C; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Division of Medical Virology, Department of Pathology, University of Cape Town, Observatory, South Africa.; Wellcome Centre for Infectious Diseases Research in Africa, University of Cape Town, Observatory, South Africa., Burgers WA; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Division of Medical Virology, Department of Pathology, University of Cape Town, Observatory, South Africa.; Wellcome Centre for Infectious Diseases Research in Africa, University of Cape Town, Observatory, South Africa., Keeton RS; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.; Division of Medical Virology, Department of Pathology, University of Cape Town, Observatory, South Africa.
Jazyk:	angličtina
Zdroj:	MedRxiv : the preprint server for health sciences [medRxiv] 2023 May 23. Date of Electronic Publication: 2023 May 23.
DOI:	10.1101/2023.05.16.23290059
Abstrakt:	SARS-CoV-2 infection in children typically results in asymptomatic or mild disease. There is a paucity of studies on antiviral immunity in African children. We investigated SARS-CoV-2-specific T cell responses in 71 unvaccinated asymptomatic South African children who were seropositive or seronegative for SARS-CoV-2. SARS-CoV-2-specific CD4+ T cell responses were detectable in 83% of seropositive and 60% of seronegative children. Although the magnitude of the CD4+ T cell response did not differ significantly between the two groups, their functional profiles were distinct, with SARS-CoV-2 seropositive children exhibiting a higher proportion of polyfunctional T cells compared to their seronegative counterparts. The frequency of SARS-CoV-2-specific CD4+ T cells in seronegative children was associated with the endemic human coronavirus (HCoV) HKU1 IgG response. Overall, the presence of SARS-CoV-2-responding T cells in seronegative children may result from cross-reactivity to endemic coronaviruses and could contribute to the relative protection from disease observed in SARS-CoV-2-infected children. Competing Interests: DECLARATION OF INTERESTS The authors have no competing interests.
Databáze:	MEDLINE
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