Resilience of A Learned Motor Behavior After Chronic Disruption of Inhibitory Circuits.

Autor:	Torok Z; Division of Biology and Biological Engineering, California Institute of Technology; Pasadena, CA, USA., Luebbert L; Division of Biology and Biological Engineering, California Institute of Technology; Pasadena, CA, USA., Feldman J; Division of Biology and Biological Engineering, California Institute of Technology; Pasadena, CA, USA., Duffy A; University of Washington; Seattle, WA, USA., Nevue AA; Oregon Health & Science University; Portland, OR, USA., Wongso S; Division of Biology and Biological Engineering, California Institute of Technology; Pasadena, CA, USA., Mello CV; Oregon Health & Science University; Portland, OR, USA., Fairhall A; University of Washington; Seattle, WA, USA., Pachter L; Division of Biology and Biological Engineering, California Institute of Technology; Pasadena, CA, USA.; Department of Computing and Mathematical Sciences, California Institute of Technology; Pasadena, CA, USA., Gonzalez WG; Department of Physiology, University of San Francisco; San Francisco, CA, USA., Lois C; Division of Biology and Biological Engineering, California Institute of Technology; Pasadena, CA, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Aug 24. Date of Electronic Publication: 2024 Aug 24.
DOI:	10.1101/2023.05.17.541057
Abstrakt:	Maintaining motor behaviors throughout life is crucial for an individual's survival and reproductive success. The neuronal mechanisms that preserve behavior are poorly understood. To address this question, we focused on the zebra finch, a bird that produces a highly stereotypical song after learning it as a juvenile. Using cell-specific viral vectors, we chronically silenced inhibitory neurons in the pre-motor song nucleus called the high vocal center (HVC), which caused drastic song degradation. However, after producing severely degraded vocalizations for around 2 months, the song rapidly improved, and animals could sing songs that highly resembled the original. In adult birds, single-cell RNA sequencing of HVC revealed that silencing interneurons elevated markers for microglia and increased expression of the Major Histocompatibility Complex I (MHC I), mirroring changes observed in juveniles during song learning. Interestingly, adults could restore their songs despite lesioning the lateral magnocellular nucleus of the anterior neostriatum (LMAN), a brain nucleus crucial for juvenile song learning. This suggests that while molecular mechanisms may overlap, adults utilize different neuronal mechanisms for song recovery. Chronic and acute electrophysiological recordings within HVC and its downstream target, the robust nucleus of the archistriatum (RA), revealed that neuronal activity in the circuit permanently altered with higher spontaneous firing in RA and lower in HVC compared to control even after the song had fully recovered. Together, our findings show that a complex learned behavior can recover despite extended periods of perturbed behavior and permanently altered neuronal dynamics. These results show that loss of inhibitory tone can be compensated for by recovery mechanisms partly local to the perturbed nucleus and do not require circuits necessary for learning. Competing Interests: Declaration of Interests The authors declare no competing interests.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu