In-silico engineering of RNA nanoplatforms to promote the diabetic wound healing.

Autor: Beheshtizadeh N; Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. n-beheshtizadeh@razi.tums.ac.ir.; Regenerative Medicine group (REMED), Universal Scientific Education and Research Network (USERN), Tehran, Iran. n-beheshtizadeh@razi.tums.ac.ir.; Students? Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran. n-beheshtizadeh@razi.tums.ac.ir., Salimi A; Regenerative Medicine group (REMED), Universal Scientific Education and Research Network (USERN), Tehran, Iran.; Department of Advanced Technologies, School of Medicine, North Khorasan University of Medical Science, Bojnurd, Iran., Golmohammadi M; Department of Polymer Engineering and Color Technology, Amirkabir University of Technology, Tehran, Iran., Ansari JM; Regenerative Medicine group (REMED), Universal Scientific Education and Research Network (USERN), Tehran, Iran.; Department of Anatomy, School of Medicine, Hormozgan University of Medical Sciences, Jomhuri Eslami Blvd, Bandar Abbas, 7919915519, Iran., Azami M; Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. m-azami@tums.ac.ir.; Regenerative Medicine group (REMED), Universal Scientific Education and Research Network (USERN), Tehran, Iran. m-azami@tums.ac.ir.
Jazyk: angličtina
Zdroj: BMC chemistry [BMC Chem] 2023 Jun 08; Vol. 17 (1), pp. 52. Date of Electronic Publication: 2023 Jun 08.
DOI: 10.1186/s13065-023-00969-4
Abstrakt: One of the most notable required features of wound healing is the enhancement of angiogenesis, which aids in the acceleration of regeneration. Poor angiogenesis during diabetic wound healing is linked to a shortage of pro-angiogenic or an increase in anti-angiogenic factors. As a result, a potential treatment method is to increase angiogenesis promoters and decrease suppressors. Incorporating microRNAs (miRNAs) and small interfering RNAs (siRNAs), two forms of quite small RNA molecules, is one way to make use of RNA interference. Several different types of antagomirs and siRNAs are now in the works to counteract the negative effects of miRNAs. The purpose of this research is to locate novel antagonists for miRNAs and siRNAs that target multiple genes to promote angiogenesis and wound healing in diabetic ulcers.In this context, we used gene ontology analysis by exploring across several datasets. Following data analysis, it was processed using a systems biology approach. The feasibility of incorporating the proposed siRNAs and miRNA antagomirs into polymeric bioresponsive nanocarriers for wound delivery was further investigated by means of a molecular dynamics (MD) simulation study. Among the three nanocarriers tested (Poly (lactic-co-glycolic acid) (PLGA), Polyethylenimine (PEI), and Chitosan (CTS), MD simulations show that the integration of PLGA/hsa-mir-422a is the most stable (total energy = -1202.62 KJ/mol, Gyration radius = 2.154 nm, and solvent-accessible surface area = 408.416 nm 2 ). With values of -25.437 KJ/mol, 0.047 nm for the Gyration radius, and 204.563 nm 2 for the SASA, the integration of the second siRNA/ Chitosan took the last place. The results of the systems biology and MD simulations show that the suggested RNA may be delivered through bioresponsive nanocarriers to speed up wound healing by boosting angiogenesis.
(© 2023. The Author(s).)
Databáze: MEDLINE
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