Long-term and low dose oral malathion exposure causes morphophysiological changes in the colon of rats.

Autor: Pupim ACE; Department of Histology, State University of Londrina, Londrina, Paraná, Brazil. Electronic address: pupim@uel.br., Basso CR; Department of Histology, State University of Londrina, Londrina, Paraná, Brazil., Machado CCA; Department of Histology, State University of Londrina, Londrina, Paraná, Brazil., Watanabe PS; Department of Histology, State University of Londrina, Londrina, Paraná, Brazil., Fernandes GSA; Department of General Biology, State University of Londrina, Londrina, Paraná, Brazil., ErthalL RP; Department of General Biology, State University of Londrina, Londrina, Paraná, Brazil., Sodré GBC; Department of Histology, State University of Londrina, Londrina, Paraná, Brazil., Guarnier FA; Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil., Simão ANC; Department of Pathology, Clinical Analysis and Toxicology, State University of Londrina, Paraná, Brazil., Araújo EJA; Department of Histology, State University of Londrina, Londrina, Paraná, Brazil.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2023 Aug 15; Vol. 327, pp. 121840. Date of Electronic Publication: 2023 Jun 07.
DOI: 10.1016/j.lfs.2023.121840
Abstrakt: Background: Malathion (MAL) is an organophosphate insecticide that inhibits cholinesterases, used to control pests in agriculture and to combat mosquitoes that transmit various arboviruses. As acetylcholine is one of the major neurotransmitters of the enteric nervous system (ENS), humans exposed to MAL by ingestion of contaminated food and water can develop symptoms due disfunction of the gastrointestinal tract. Although the deleterious effects after exposure to high doses are recognized, little is known about the long-term and low-dose effects of this pesticide on the structure and motility of the colon.
Aims: to evaluate the effects of prolonged oral exposure to low levels of MAL on the wall structure and colonic motility parameters of young rats.
Main Methods: The animals were divided into three groups: control, and groups that received 10 or 50 mg/kg of MAL via gavage for 40 days. The colon was collected for histological analysis and analysis of the ENS through the evaluation of total neurons and subpopulations of the myenteric and submucosal plexuses. Cholinesterase activity and functional analyzes of the colon were evaluated.
Key Findings: MAL treatments (10 and 50 mg/Kg) reduced the butyrylcholinesterase activity, and caused enlargement of faecal pellets, atrophy of muscle layers and several changes in neurons of both myenteric and submucosal plexi. Considering colonic contraction, MAL (50 mg/Kg) increased the number of retrograde colonic migratory motor complexes.
Significance: The long-term exposure to low doses of MAL affects colonic morphophysiology, which highlights the need to intensify control and care in the use of this pesticide.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: