Turkey adenovirus 3: ORF1 gene sequence comparison between vaccine-like and field strains.

Autor: Quaglia G; Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50, Ozzano dell'Emilia (BO), 40064, Italy. giulia.quaglia2@unibo.it., Di Francesco A; Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50, Ozzano dell'Emilia (BO), 40064, Italy., Catelli E; Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50, Ozzano dell'Emilia (BO), 40064, Italy., Mescolini G; Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50, Ozzano dell'Emilia (BO), 40064, Italy., Lupini C; Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50, Ozzano dell'Emilia (BO), 40064, Italy.
Jazyk: angličtina
Zdroj: Veterinary research communications [Vet Res Commun] 2023 Dec; Vol. 47 (4), pp. 2307-2313. Date of Electronic Publication: 2023 Jun 08.
DOI: 10.1007/s11259-023-10148-4
Abstrakt: Haemorrhagic enteritis is an economically significant disease reported in the majority of the countries where turkeys are raised intensively; it is caused by Turkey adenovirus 3 (TAdV-3). The aim of this study was to analyse and compare the ORF1 gene 3' region from turkey haemorrhagic enteritis virus (THEV) vaccine-like and field strains in order to develop a molecular diagnostic method to differentiate the strains from each other. Eighty samples were analysed by sequencing and phylogenetic analyses using a new set of polymerase chain reaction (PCR) primers targeting a genomic region spanning the partial ORF1, hyd and partial IVa2 gene sequences. A commercial live vaccine was also included in the analysis. The results showed that 56 of the 80 sequences obtained in this study showed ≥99.8% nucleotide identity with the homologous vaccine strain sequence. Three non-synonymous mutations - ntA1274G (aaI425V), ntA1420C (aaQ473H) and ntG1485A (aaR495Q) - were detected in the THEV field strains but not in the vaccine strain. Phylogenetic analysis confirmed the clustering of the field and vaccine-like strains in different phylogenetic branches. In conclusion, the method employed in this study could be a useful tool towards making a correct diagnosis. The data could contribute to the knowledge of field distribution of THEV strains and increase the limited existing information available on native isolates around the world.
(© 2023. The Author(s).)
Databáze: MEDLINE
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