A systems serology approach to the investigation of infection-induced antibody responses and protection in trachoma.

Autor:	Barton A; Department of Clinical Research, London School of Hygiene and Tropical Medicine, London, United Kingdom., Rosenkrands I; Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark., Pickering H; Department of Clinical Research, London School of Hygiene and Tropical Medicine, London, United Kingdom., Faal N; Medical Research Council Unit The Gambia at London School of Hygiene and Tropical Medicine, Banjul, Gambia., Harte A; Department of Clinical Research, London School of Hygiene and Tropical Medicine, London, United Kingdom., Joof H; Medical Research Council Unit The Gambia at London School of Hygiene and Tropical Medicine, Banjul, Gambia., Makalo P; Medical Research Council Unit The Gambia at London School of Hygiene and Tropical Medicine, Banjul, Gambia., Ragonnet M; Medical Research Council Unit The Gambia at London School of Hygiene and Tropical Medicine, Banjul, Gambia., Olsen AW; Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark., Bailey RL; Department of Clinical Research, London School of Hygiene and Tropical Medicine, London, United Kingdom., Mabey DCW; Department of Clinical Research, London School of Hygiene and Tropical Medicine, London, United Kingdom., Follmann F; Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark., Dietrich J; Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark., Holland MJ; Department of Clinical Research, London School of Hygiene and Tropical Medicine, London, United Kingdom.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2023 May 23; Vol. 14, pp. 1178741. Date of Electronic Publication: 2023 May 23 (Print Publication: 2023).
DOI:	10.3389/fimmu.2023.1178741
Abstrakt:	Background: Ocular infections with Chlamydia trachomatis serovars A-C cause the neglected tropical disease trachoma. As infection does not confer complete immunity, repeated infections are common, leading to long-term sequelae such as scarring and blindness. Here, we apply a systems serology approach to investigate whether systemic antibody features are associated with susceptibility to infection. Methods: Sera from children in five trachoma endemic villages in the Gambia were assayed for 23 antibody features: IgG responses towards two C. trachomatis antigens and three serovars [elementary bodies and major outer membrane protein (MOMP), serovars A-C], IgG responses towards five MOMP peptides (serovars A-C), neutralization, and antibody-dependent phagocytosis. Participants were considered resistant if they subsequently developed infection only when over 70% of other children in the same compound were infected. Results: The antibody features assayed were not associated with resistance to infection (false discovery rate < 0.05). Anti-MOMP SvA IgG and neutralization titer were higher in susceptible individuals ( p < 0.05 before multiple testing adjustment). Classification using partial least squares performed only slightly better than chance in distinguishing between susceptible and resistant participants based on systemic antibody profile (specificity 71%, sensitivity 36%). Conclusions: Systemic infection-induced IgG and functional antibody responses do not appear to be protective against subsequent infection. Ocular responses, IgA, avidity, or cell-mediated responses may play a greater role in protective immunity than systemic IgG. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Barton, Rosenkrands, Pickering, Faal, Harte, Joof, Makalo, Ragonnet, Olsen, Bailey, Mabey, Follmann, Dietrich and Holland.)
Databáze:	MEDLINE
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