Scattered-light-sheet microscopy with sub-cellular resolving power.

Autor: Subedi NR; Department of Physics, University of Idaho, Moscow, Idaho, USA., Stolyar S; Department of Biological Sciences, University of Idaho, Moscow, Idaho, USA., Tuson SJ; Department of Physics, University of Idaho, Moscow, Idaho, USA., Marx CJ; Department of Biological Sciences, University of Idaho, Moscow, Idaho, USA., Vasdekis AE; Department of Physics, University of Idaho, Moscow, Idaho, USA.
Jazyk: angličtina
Zdroj: Journal of biophotonics [J Biophotonics] 2023 Sep; Vol. 16 (9), pp. e202300068. Date of Electronic Publication: 2023 Jun 20.
DOI: 10.1002/jbio.202300068
Abstrakt: Since its first demonstration over 100 years ago, scattering-based light-sheet microscopy has recently re-emerged as a key modality in label-free tissue imaging and cellular morphometry; however, scattering-based light-sheet imaging with subcellular resolution remains an unmet target. This is because related approaches inevitably superimpose speckle or granular intensity modulation on to the native subcellular features. Here, we addressed this challenge by deploying a time-averaged pseudo-thermalized light-sheet illumination. While this approach increased the lateral dimensions of the illumination sheet, we achieved subcellular resolving power after image deconvolution. We validated this approach by imaging cytosolic carbon depots in yeast and bacteria with increased specificity, no staining, and ultralow irradiance levels. Overall, we expect this scattering-based light-sheet microscopy approach will advance single, live cell imaging by conferring low-irradiance and label-free operation towards eradicating phototoxicity.
(© 2023 Wiley-VCH GmbH.)
Databáze: MEDLINE
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