Variations in Intracellular Organometallic Reaction Frequency Captured by Single-Molecule Fluorescence Microscopy.

Autor: Nguyen D; Faculty of Chemical and Food Technology, Ho Chi Minh City University of Technology and Education, 1 Vo Van Ngan, Thu Duc City, Ho Chi Minh City, Vietnam., Yan G; Department of Chemistry, University of Houston, 4800 Calhoun Rd., TX 77004, Houston, USA., Chen TY; Department of Chemistry, University of Houston, 4800 Calhoun Rd., TX 77004, Houston, USA., Do LH; Department of Chemistry, University of Houston, 4800 Calhoun Rd., TX 77004, Houston, USA.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2023 Aug 01; Vol. 62 (31), pp. e202300467. Date of Electronic Publication: 2023 Jun 27.
DOI: 10.1002/anie.202300467
Abstrakt: Studies of organometallic reactions in living cells commonly rely on ensemble-averaged measurements, which can obscure the detection of reaction dynamics or location-specific behavior. This information is necessary to guide the design of bioorthogonal catalysts with improved biocompatibility, activity, and selectivity. By leveraging the high spatial and temporal resolution of single-molecule fluorescence microscopy, we have successfully captured single-molecule events promoted by Ru complexes inside live A549 human lung cells. By observing individual allylcarbamate cleavage reactions in real-time, our results revealed that they occur with greater frequency inside the mitochondria than in the non-mitochondria regions. The estimated turnover frequency of the Ru complexes was at least 3-fold higher in the former than the latter. These results suggest that organelle specificity is a critical factor to consider in intracellular catalyst design, such as in developing metallodrugs for therapeutic applications.
(© 2023 Wiley-VCH GmbH.)
Databáze: MEDLINE
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