Novel viruses of the family Partitiviridae discovered in Saccharomyces cerevisiae.

Autor: Taggart NT; Department of Biological Sciences, University of Idaho, Moscow, Idaho, United States of America., Crabtree AM; Department of Biological Sciences, University of Idaho, Moscow, Idaho, United States of America., Creagh JW; Department of Biological Sciences, University of Idaho, Moscow, Idaho, United States of America., Bizarria R Jr; Department of Biological Sciences, University of Idaho, Moscow, Idaho, United States of America.; Department of General and Applied Biology, São Paulo State University (UNESP), Rio Claro, São Paulo, Brazil.; Center for the Study of Social Insects, São Paulo State University (UNESP), Rio Claro, São Paulo, Brazil., Li S; Department of Biological Sciences, University of Idaho, Moscow, Idaho, United States of America., de la Higuera I; Center for Life in Extreme Environments, Department of Biology, Portland State University, Portland, Oregon, United States of America., Barnes JE; Institute for Modeling Collaboration and Innovation, University of Idaho, Moscow, Idaho, United States of America., Shipley MA; Department of Biological Sciences, University of Idaho, Moscow, Idaho, United States of America., Boyer JM; Department of Biological Sciences, University of Idaho, Moscow, Idaho, United States of America., Stedman KM; Center for Life in Extreme Environments, Department of Biology, Portland State University, Portland, Oregon, United States of America., Ytreberg FM; Institute for Modeling Collaboration and Innovation, University of Idaho, Moscow, Idaho, United States of America.; Department of Physics, University of Idaho, Moscow, Idaho, United States of America., Rowley PA; Department of Biological Sciences, University of Idaho, Moscow, Idaho, United States of America.
Jazyk: angličtina
Zdroj: PLoS pathogens [PLoS Pathog] 2023 Jun 07; Vol. 19 (6), pp. e1011418. Date of Electronic Publication: 2023 Jun 07 (Print Publication: 2023).
DOI: 10.1371/journal.ppat.1011418
Abstrakt: It has been 49 years since the last discovery of a new virus family in the model yeast Saccharomyces cerevisiae. A large-scale screen to determine the diversity of double-stranded RNA (dsRNA) viruses in S. cerevisiae has identified multiple novel viruses from the family Partitiviridae that have been previously shown to infect plants, fungi, protozoans, and insects. Most S. cerevisiae partitiviruses (ScPVs) are associated with strains of yeasts isolated from coffee and cacao beans. The presence of partitiviruses was confirmed by sequencing the viral dsRNAs and purifying and visualizing isometric, non-enveloped viral particles. ScPVs have a typical bipartite genome encoding an RNA-dependent RNA polymerase (RdRP) and a coat protein (CP). Phylogenetic analysis of ScPVs identified three species of ScPV, which are most closely related to viruses of the genus Cryspovirus from the mammalian pathogenic protozoan Cryptosporidium parvum. Molecular modeling of the ScPV RdRP revealed a conserved tertiary structure and catalytic site organization when compared to the RdRPs of the Picornaviridae. The ScPV CP is the smallest so far identified in the Partitiviridae and has structural homology with the CP of other partitiviruses but likely lacks a protrusion domain that is a conspicuous feature of other partitivirus particles. ScPVs were stably maintained during laboratory growth and were successfully transferred to haploid progeny after sporulation, which provides future opportunities to study partitivirus-host interactions using the powerful genetic tools available for the model organism S. cerevisiae.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Taggart et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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