| Autor: |
Souza PMS; Universidade de Pernambuco - UPE, Instituto de Ciências Biológicas, Laboratório de Resistência Microbiana, Recife, PE, Brasil., Ribeiro ACOA; Universidade de Pernambuco - UPE, Instituto de Ciências Biológicas, Laboratório de Resistência Microbiana, Recife, PE, Brasil., Pena EPN; Universidade Federal de Pernambuco - UFPE, Departamento de Genética, Laboratório de Genômica e Proteômica de Plantas, Recife, PE, Brasil., Silva FAC; Universidade Federal de Pernambuco - UFPE, Departamento de Genética, Laboratório de Genômica e Proteômica de Plantas, Recife, PE, Brasil., Calsa Júnior T; Universidade Federal de Pernambuco - UFPE, Departamento de Genética, Laboratório de Genômica e Proteômica de Plantas, Recife, PE, Brasil., Morais MMC; Universidade de Pernambuco - UPE, Instituto de Ciências Biológicas, Laboratório de Resistência Microbiana, Recife, PE, Brasil., Almeida ACS; Universidade Federal Rural de Pernambuco - UFRPE, Departamento de Biologia, Laboratório de Genética, Bioquímica e Sequenciamento de DNA, Recife, PE, Brasil. |
| Abstrakt: |
The isolation of multidrug-resistant Klebsiella pneumoniae in hospitals is a major public health threat, increasing patient hospitalization costs, morbidity and mortality. Therefore, this work investigated the resistance mechanisms that produced different carbapenems susceptibility profiles in two isogenic strains of K. pneumoniae isolated from the same patient in a public hospital in Recife, Pernambuco. The genes that encode the main porins in K. pneumoniae, ompK35 and ompK36, and several beta-lactamase genes were analyzed. The expression of these genes was evaluated by quantitative real time PCR (polymerase chain reaction) with reverse transcriptase (RT-qPCR). SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) was performed to analyze the outer membrane proteins. The analysis of the ompK36 genetic environment disclosed an IS903 insertion sequence disrupting this gene in the ertapenem resistant isolate (KPN133). The blaKPC-2 gene showed down-regulated expression in both isolates. Our findings show that changes in porins, especially OmpK36, are more determinant to carbapenems susceptibility profile of bacterial isolates than variations in blaKPC gene expression. |
