Testosterone and estradiol modify the expression of adhesins and biofilm formation in Actinobacillus seminis.

Autor:	Ramírez-Paz-Y-Puente GA; Facultad de Estudios Superiores Iztacala, UNAM,Av. De Los Barrios 1, Los Reyes Iztacala, 54090 Tlalnepantla, Edo de México, Mexico., Chávez-Flores CI; Facultad de Estudios Superiores Iztacala, UNAM,Av. De Los Barrios 1, Los Reyes Iztacala, 54090 Tlalnepantla, Edo de México, Mexico., Montes-García JF; Facultad de Estudios Superiores Iztacala, UNAM,Av. De Los Barrios 1, Los Reyes Iztacala, 54090 Tlalnepantla, Edo de México, Mexico., Sanchez-Alonso PG; Centro de Investigaciones en Ciencias Microbiológicas, BUAP, Apdo, 1622, Puebla, 72560, Puebla, Mexico., Cobos-Justo ME; Centro de Investigaciones en Ciencias Microbiológicas, BUAP, Apdo, 1622, Puebla, 72560, Puebla, Mexico., Vázquez-Cruz C; Centro de Investigaciones en Ciencias Microbiológicas, BUAP, Apdo, 1622, Puebla, 72560, Puebla, Mexico., Zenteno E; Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Av. Universidad 3000, CU, 04510, Coyoacan Ciudad de México, Mexico., Negrete-Abascal E; Facultad de Estudios Superiores Iztacala, UNAM,Av. De Los Barrios 1, Los Reyes Iztacala, 54090 Tlalnepantla, Edo de México, Mexico.
Jazyk:	angličtina
Zdroj:	FEMS microbiology letters [FEMS Microbiol Lett] 2023 Jan 17; Vol. 370.
DOI:	10.1093/femsle/fnad048
Abstrakt:	Actinobacillus seminis is the causal agent of epididymitis and has other effects on the reproductive tracts of small ruminants and bovines. This bacterium causes infection when luteinizing (LH) or follicle-stimulating hormones increase, and hosts reach sexual maturity. LH induces female ovulation and male testosterone production, suggesting that these hormones affect A. seminis pathogenicity. In the present study, we evaluated the effect of testosterone (1-5 ng/ml) or estradiol (5-25 pg/ml) added to culture medium on the in vitro growth, biofilm production, and adhesin expression of A. seminis. Estradiol does not promote the growth of this bacterium, whereas testosterone increased A. seminis planktonic growth 2-fold. Both hormones induced the expression of the elongation factor thermo unstable (EF-Tu) and phosphoglycerate mutase (PGM), proteins that A. seminis uses as adhesins. Estradiol (5 or 10 pg/ml) decreased biofilm formation by 32%, whereas testosterone, even at 5 ng/ml, showed no effect. Both hormones modified the concentrations of carbohydrates and eDNA in biofilms by 50%. Amyloid proteins are characterized by their capacity to bind Congo red (CR) dye. Actinobacillus seminis binds CR dye, and this binding increases in the presence of 5-20 pg/ml estradiol or 4 ng/ml testosterone. The A. seminis EF-Tu protein was identified as amyloid-like protein (ALP). The effect of sexual hormones on the growth and expression of virulence factors of A. seminis seems to be relevant for its colonization and permanence in the host. (© The Author(s) 2023. Published by Oxford University Press on behalf of FEMS.)
Databáze:	MEDLINE
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