Recent progress in CRISPR-based bioengineering of microbial cell factories for important nutraceuticals synthesis.

Autor:	Hussain MI; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.; Science Center for Future Foods, Jiangnan University, Wuxi 214122, China., Raziq A; State Key Laboratory for Biology of Plant Diseases and Insect Pests, School of Bioengineering, Dalian University of Technology, Dalian 116024, China., Ahmed A; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China., Iqbal MW; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China., Tian R; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.; Science Center for Future Foods, Jiangnan University, Wuxi 214122, China., Li J; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.; Science Center for Future Foods, Jiangnan University, Wuxi 214122, China., Liu L; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.; Science Center for Future Foods, Jiangnan University, Wuxi 214122, China., Liu Y; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.; Science Center for Future Foods, Jiangnan University, Wuxi 214122, China.
Jazyk:	angličtina
Zdroj:	Journal of applied microbiology [J Appl Microbiol] 2023 Jun 01; Vol. 134 (6).
DOI:	10.1093/jambio/lxad114
Abstrakt:	Nutraceuticals are defined as food or food components with therapeutic capabilities that have few side effects and are regarded as a natural therapy for preventing the onset of several life-threatening illnesses. The use of microbial cell factories to produce nutraceuticals is considered to be sustainable and promising for meeting market demand. Among the diverse strategies for optimizing microbial cell factories, the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system has emerged as a valuable tool for gene integration, deletion, activation, and downregulation. With the advent of multiplexed and precise CRISPR strategies, optimized microbial cell factories are revolutionizing the yield of nutraceuticals. This review focuses on the development of highly adaptable CRISPR strategies to optimize the production in microbial cell factories of some important nutraceuticals (belonging to the class of carotenoids, flavonoids, stilbenoids, polysaccharides, and nonprotein amino acids). Further, we highlighted current challenges related to the efficiency of CRISPR strategies and addressed potential future directions to fully harness CRISPR strategies to make nutraceutical synthesis in microbial cell factories an industrially favorable method. (© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.)
Databáze:	MEDLINE
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