Local translation in microglial processes is required for efficient phagocytosis.

Autor: Vasek MJ; Department of Genetics, Washington University School of Medicine, Saint Louis, MO, USA.; Department of Psychiatry, Washington University School of Medicine, Saint Louis, MO, USA., Mueller SM; Department of Genetics, Washington University School of Medicine, Saint Louis, MO, USA.; Department of Psychiatry, Washington University School of Medicine, Saint Louis, MO, USA., Fass SB; Department of Genetics, Washington University School of Medicine, Saint Louis, MO, USA.; Department of Psychiatry, Washington University School of Medicine, Saint Louis, MO, USA., Deajon-Jackson JD; Department of Genetics, Washington University School of Medicine, Saint Louis, MO, USA.; Department of Psychiatry, Washington University School of Medicine, Saint Louis, MO, USA., Liu Y; Department of Genetics, Washington University School of Medicine, Saint Louis, MO, USA.; Department of Psychiatry, Washington University School of Medicine, Saint Louis, MO, USA., Crosby HW; Department of Genetics, Washington University School of Medicine, Saint Louis, MO, USA.; Department of Psychiatry, Washington University School of Medicine, Saint Louis, MO, USA., Koester SK; Department of Genetics, Washington University School of Medicine, Saint Louis, MO, USA.; Department of Psychiatry, Washington University School of Medicine, Saint Louis, MO, USA.; Division of Biology and Biomedical Sciences, Washington University School of Medicine, Saint Louis, MO, USA., Yi J; Department of Genetics, Washington University School of Medicine, Saint Louis, MO, USA.; Department of Psychiatry, Washington University School of Medicine, Saint Louis, MO, USA.; Division of Biology and Biomedical Sciences, Washington University School of Medicine, Saint Louis, MO, USA., Li Q; Department of Genetics, Washington University School of Medicine, Saint Louis, MO, USA.; Department of Neuroscience, Washington University School of Medicine, Saint Louis, MO, USA., Dougherty JD; Department of Genetics, Washington University School of Medicine, Saint Louis, MO, USA. jdougherty@wustl.edu.; Department of Psychiatry, Washington University School of Medicine, Saint Louis, MO, USA. jdougherty@wustl.edu.
Jazyk: angličtina
Zdroj: Nature neuroscience [Nat Neurosci] 2023 Jul; Vol. 26 (7), pp. 1185-1195. Date of Electronic Publication: 2023 Jun 05.
DOI: 10.1038/s41593-023-01353-0
Abstrakt: Neurons, astrocytes and oligodendrocytes locally regulate protein translation within distal processes. Here, we tested whether there is regulated local translation within peripheral microglial processes (PeMPs) from mouse brain. We show that PeMPs contain ribosomes that engage in de novo protein synthesis, and these are associated with transcripts involved in pathogen defense, motility and phagocytosis. Using a live slice preparation, we further show that acute translation blockade impairs the formation of PeMP phagocytic cups, the localization of lysosomal proteins within them, and phagocytosis of apoptotic cells and pathogen-like particles. Finally, PeMPs severed from their somata exhibit and require de novo local protein synthesis to effectively surround pathogen-like particles. Collectively, these data argue for regulated local translation in PeMPs and indicate a need for new translation to support dynamic microglial functions.
(© 2023. The Author(s), under exclusive licence to Springer Nature America, Inc.)
Databáze: MEDLINE
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