In vivo anticoccidial activity and immune response of Moringa oleifera and Vernonia amygdalina leaves against Eimeria magna and Eimeria media in rabbits.

Autor:	Konmy B; Zootechnical Research and Livestock System Unit, School of Management and Operation of Livestock Systems, National University of Agriculture, Porto-Novo, Benin.; Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Techniques, University of Abomey-Calavi, Abomey-Calavi, Benin.; Laboratory of Ethnopharmacology and Animal Health, Faculty of Agronomic Sciences, University of Abomey-Calavi, Cotonou, Benin., Olounladé PA; Zootechnical Research and Livestock System Unit, School of Management and Operation of Livestock Systems, National University of Agriculture, Porto-Novo, Benin.; Laboratory of Ethnopharmacology and Animal Health, Faculty of Agronomic Sciences, University of Abomey-Calavi, Cotonou, Benin., Adjobimey T; Unité de Biochimie et de Biologie Moléculaire, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, Abomey-Calavi, Benin., Dansou CC; Zootechnical Research and Livestock System Unit, School of Management and Operation of Livestock Systems, National University of Agriculture, Porto-Novo, Benin., Adoho ACC; Zootechnical Research and Livestock System Unit, School of Management and Operation of Livestock Systems, National University of Agriculture, Porto-Novo, Benin., Tchetan E; Zootechnical Research and Livestock System Unit, School of Management and Operation of Livestock Systems, National University of Agriculture, Porto-Novo, Benin.; Laboratory of Ethnopharmacology and Animal Health, Faculty of Agronomic Sciences, University of Abomey-Calavi, Cotonou, Benin., Alowanou GG; Laboratory of Ethnopharmacology and Animal Health, Faculty of Agronomic Sciences, University of Abomey-Calavi, Cotonou, Benin., Azando EVB; Laboratory of Ecology, Health and Animal Production, Faculty of Agronomy, University of Parakou, Parakou, Benin., Adjanohoun A; Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Techniques, University of Abomey-Calavi, Abomey-Calavi, Benin., Doko Allou SY; Zootechnical Research and Livestock System Unit, School of Management and Operation of Livestock Systems, National University of Agriculture, Porto-Novo, Benin., Baba-Moussa L; Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Techniques, University of Abomey-Calavi, Abomey-Calavi, Benin.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2023 May 18; Vol. 13, pp. 1173138. Date of Electronic Publication: 2023 May 18 (Print Publication: 2023).
DOI:	10.3389/fcimb.2023.1173138
Abstrakt:	Introduction: The aims of this study were to determine the immune response and the anticoccidial activity induced by Moringa oleifera and Vernonia amygdalina leaves in rabbits infected with Eimeria magna and Eimeria media . Methods: Thirty-five-day-old rabbits, free from coccidia, were infested with 2.10 3 oocysts of Eimeria magna and Eimeria media , then received the acetone extract of the leaves of Moringa oleifera and Vernonia amygdalina at different doses by oral gavage. Results and Discussion: The inhibition of the excretion of oocysts was evaluated by the McMaster technique and the levels of cytokines (IL-4 and IL-12) and immunoglobulin IgG were assayed by the ELISA method. The in vivo efficacy on E. magna and E. media oocysts was 95.43% and 96.53% for Moringa oleifera and Vernonia amygdalina at 1000 mg/kg bw against 98% for the positive control. Interestingly the plant extracts increased the production of interleukin (IL) and immunoglobulins (Ig) compared to controls. Plasma IL-4 levels (pg/ml) in rabbits were 128.94 and 131.38; those of IL-12 (pg/ml) were 395.55 and 426.56, and then for those of IgG (μg/ml) were 14.70 and 13.94 respectively with the acetone extracts of Moringa oleifera and Vernonia amygdalina on D14 PT at 1000 mg/kg bw. This study indicates that Moringa oleifera and Vernonia amygdalina can be used as an alternative to synthetic anticoccidials. These plants could be used to increase the resistance of the immune system of rabbits to infestations of Eimeria species in rabbit farms. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Konmy, Olounladé, Adjobimey, Dansou, Adoho, Tchetan, Alowanou, Azando, Adjanohoun, Doko Allou and Baba-Moussa.)
Databáze:	MEDLINE
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