Sequence Divergence in Venom Genes Within and Between Montane Pitviper (Viperidae: Crotalinae: Cerrophidion) Species is Driven by Mutation-Drift Equilibrium.

Autor: Rosales-García RA; Department of Biological Sciences, Clemson University, 190 Collings St., Clemson, SC, 29634, USA. ramsesr@g.clemson.edu., Rautsaw RM; Department of Biological Sciences, Clemson University, 190 Collings St., Clemson, SC, 29634, USA., Hofmann EP; Department of Biological Sciences, Clemson University, 190 Collings St., Clemson, SC, 29634, USA.; Science Department, Cape Fear Community College, Wilmington, NC, 28401, USA., Grünwald CI; Herp.mx A.C., Colima, Mexico.; Biodiversa A. C., Chapala, Jalisco, 45900, Mexico., Franz-Chavez H; Herp.mx A.C., Colima, Mexico.; Biodiversa A. C., Chapala, Jalisco, 45900, Mexico., Ahumada-Carrillo IT; Herp.mx A.C., Colima, Mexico.; Biodiversa A. C., Chapala, Jalisco, 45900, Mexico., Ramirez-Chaparro R; Herp.mx A.C., Colima, Mexico.; Biodiversa A. C., Chapala, Jalisco, 45900, Mexico., de la Torre-Loranca MA; Instituto Lorancai, Ocotepec, Veracruz, 24105, Mexico., Strickland JL; Department of Biological Sciences, Clemson University, 190 Collings St., Clemson, SC, 29634, USA.; Department of Biology, University of South Alabama, Mobile, AL, 36688, USA., Mason AJ; Department of Biological Sciences, Clemson University, 190 Collings St., Clemson, SC, 29634, USA.; Department of Evolution, Ecology, and Organismal Biology, The Ohio State University, Columbus, OH, 43210, USA., Holding ML; Department of Biological Sciences, Clemson University, 190 Collings St., Clemson, SC, 29634, USA.; Life Sciences Institute, University of Michigan, Ann Arbor, MI, 48109, USA., Borja M; Facultad de Ciencias Biológicas, Universdad Juárez del Estado de Durango, Gómez Palacio, Durango, 35010, Mexico., Castañeda-Gaytan G; Facultad de Ciencias Biológicas, Universdad Juárez del Estado de Durango, Gómez Palacio, Durango, 35010, Mexico., Myers EA; Department of Biological Sciences, Clemson University, 190 Collings St., Clemson, SC, 29634, USA., Sasa M; Centro Investigaciones en Biodiversidad y Ecología Tropical and Instituto Clodomiro Picado, Universidad de Costa Rica, San José, Costa Rica., Rokyta DR; Department of Biological Science, Florida State University, Tallahassee, FL, 32306, USA., Parkinson CL; Department of Biological Sciences, Clemson University, 190 Collings St., Clemson, SC, 29634, USA. viper@clemson.edu.
Jazyk: angličtina
Zdroj: Journal of molecular evolution [J Mol Evol] 2023 Aug; Vol. 91 (4), pp. 514-535. Date of Electronic Publication: 2023 Jun 03.
DOI: 10.1007/s00239-023-10115-2
Abstrakt: Snake venom can vary both among and within species. While some groups of New World pitvipers-such as rattlesnakes-have been well studied, very little is known about the venom of montane pitvipers (Cerrophidion) found across the Mesoamerican highlands. Compared to most well-studied rattlesnakes, which are widely distributed, the isolated montane populations of Cerrophidion may facilitate unique evolutionary trajectories and venom differentiation. Here, we describe the venom gland transcriptomes for populations of C. petlalcalensis, C. tzotzilorum, and C. godmani from Mexico, and a single individual of C. sasai from Costa Rica. We explore gene expression variation in Cerrophidion and sequence evolution of toxins within C. godmani specifically. Cerrophidion venom gland transcriptomes are composed primarily of snake venom metalloproteinases, phospholipase A[Formula: see text]s (PLA[Formula: see text]s), and snake venom serine proteases. Cerrophidion petlalcalensis shows little intraspecific variation; however, C. godmani and C. tzotzilorum differ significantly between geographically isolated populations. Interestingly, intraspecific variation was mostly attributed to expression variation as we did not detect signals of selection within C. godmani toxins. Additionally, we found PLA[Formula: see text]-like myotoxins in all species except C. petlalcalensis, and crotoxin-like PLA[Formula: see text]s in the southern population of C. godmani. Our results demonstrate significant intraspecific venom variation within C. godmani and C. tzotzilorum. The toxins of C. godmani show little evidence of directional selection where variation in toxin sequence is consistent with evolution under a model of mutation-drift equilibrium. Cerrophidion godmani individuals from the southern population may exhibit neurotoxic venom activity given the presence of crotoxin-like PLA[Formula: see text]s; however, further research is required to confirm this hypothesis.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje