Roles of qseC mutation in bacterial resistance against anti-lipopolysaccharide factor isoform 3 (ALFPm3).

Autor: Khunsri I; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.; Center for Vaccine Development, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand., Prombutara P; Omics Science and Bioinformatics Center, Faculty of Science, Chulalongkorn University, Bangkok, Thailand., Htoo HH; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand., Wanvimonsuk S; Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand., Samernate T; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand., Pornsing C; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand., Tharntada S; Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand., Jaree P; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand., Chaikeeratisak V; Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand., Somboonwiwat K; Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand., Nonejuie P; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Jun 02; Vol. 18 (6), pp. e0286764. Date of Electronic Publication: 2023 Jun 02 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0286764
Abstrakt: Propelled by global climate changes, the shrimp industry has been facing tremendous losses in production due to various disease outbreaks, particularly early mortality syndrome (EMS), a disease caused by Vibrio parahaemolyticus AHPND. Not only is the use of antibiotics as EMS control agents not yet been proven successful, but the overuse and misuse of antibiotics could also worsen one of the most challenging global health issues-antimicrobial resistance. To circumvent antibiotic usage, anti-lipopolysaccharide factor isoform 3 (ALFPm3), an antimicrobial peptide (AMP) derived from the shrimp innate immune system, was proposed as an antibiotic alternative for EMS control. However, prolonged use of AMPs could also lead to bacterial cross resistance with life-saving antibiotics used in human diseases. Here, we showed that ALFPm3-resistant strains of E. coli could be induced in vitro. Genome analysis of the resistant mutants revealed multiple mutations, with the most interesting being a qseC(L299R). A study of antibiotic susceptibility profile showed that the resistant strains harboring the qseC(L299R) not only exhibited higher degree of resistance towards polymyxin antibiotics, but also produced higher biofilm under ALFPm3 stress. Lastly, a single cell death analysis revealed that, at early-log phase when biofilm is scarce, the resistant strains were less affected by ALFPm3 treatment, suggesting additional mechanisms by which qseC orchestrates to protect the bacteria from ALFPm3. Altogether, this study uncovers involvement of qseC mutation in mechanism of resistance of the bacteria against ALFPm3 paving a way for future studies on sustainable use of ALFPm3 as an EMS control agent.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Khunsri et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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