| Autor: |
Chen Y; School of Science, Constructor University, Campus Ring 1, 28759 Bremen, Germany., Barba-Bon A; School of Science, Constructor University, Campus Ring 1, 28759 Bremen, Germany., Grüner B; Institute of Inorganic Chemistry, Czech Academy of Sciences, v.v.i. Hlavní 1001, CZ-250 68 Řež, Czech Republic., Winterhalter M; School of Science, Constructor University, Campus Ring 1, 28759 Bremen, Germany., Aksoyoglu MA; School of Science, Constructor University, Campus Ring 1, 28759 Bremen, Germany., Pangeni S; School of Science, Constructor University, Campus Ring 1, 28759 Bremen, Germany., Ashjari M; School of Science, Constructor University, Campus Ring 1, 28759 Bremen, Germany., Brix K; School of Science, Constructor University, Campus Ring 1, 28759 Bremen, Germany., Salluce G; Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS), Universidade de Santiago de Compostela, Jenaro de la Fuente s/n, 15782 Santiago de Compostela, Spain., Folgar-Cameán Y; Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS), Universidade de Santiago de Compostela, Jenaro de la Fuente s/n, 15782 Santiago de Compostela, Spain., Montenegro J; Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS), Universidade de Santiago de Compostela, Jenaro de la Fuente s/n, 15782 Santiago de Compostela, Spain., Nau WM; School of Science, Constructor University, Campus Ring 1, 28759 Bremen, Germany. |
| Abstrakt: |
Cobalt bisdicarbollides (COSANs) are inorganic boron-based anions that have been previously reported to permeate by themselves through lipid bilayer membranes, a propensity that is related to their superchaotropic character. We now introduce their use as selective and efficient molecular carriers of otherwise impermeable hydrophilic oligopeptides through both artificial and cellular membranes, without causing membrane lysis or poration at low micromolar carrier concentrations. COSANs transport not only arginine-rich but also lysine-rich peptides, whereas low-molecular-weight analytes such as amino acids as well as neutral and anionic cargos (phalloidin and BSA) are not transported. In addition to the unsubstituted isomers (known as ortho - and meta -COSAN), four derivatives bearing organic substituents or halogen atoms have been evaluated, and all six of them surpass established carriers such as pyrenebutyrate in terms of activity. U-tube experiments and black lipid membrane conductance measurements establish that the transport across model membranes is mediated by a molecular carrier mechanism. Transport experiments in living cells showed that a fluorescent peptide cargo, FITC-Arg 8 , is delivered into the cytosol. |
