A functional group-guided approach to aptamers for small molecules.

Autor: Yang K; Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA., Mitchell NM; Department of Chemistry and Biochemistry and California Nanosystems Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA., Banerjee S; Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA., Cheng Z; Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA., Taylor S; Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA., Kostic AM; Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA., Wong I; Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA., Sajjath S; Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA., Zhang Y; Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA., Stevens J; Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA., Mohan S; Department of Epidemiology, Mailman School of Public Health, New York, NY 10032, USA., Landry DW; Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA., Worgall TS; Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, NY 10032, USA., Andrews AM; Department of Chemistry and Biochemistry and California Nanosystems Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA.; Department of Psychiatry and Biobehavioral Sciences and Hatos Center for Neuropharmacology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA., Stojanovic MN; Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA.; Departments of Biomedical Engineering, Fu Foundation School of Engineering and Applied Science, and Systems Biology, Columbia University Irving Medical Center, New York, NY 10032, USA.
Jazyk: angličtina
Zdroj: Science (New York, N.Y.) [Science] 2023 Jun 02; Vol. 380 (6648), pp. 942-948. Date of Electronic Publication: 2023 Jun 01.
DOI: 10.1126/science.abn9859
Abstrakt: Aptameric receptors are important biosensor components, yet our ability to identify them depends on the target structures. We analyzed the contributions of individual functional groups on small molecules to binding within 27 target-aptamer pairs, identifying potential hindrances to receptor isolation-for example, negative cooperativity between sterically hindered functional groups. To increase the probability of aptamer isolation for important targets, such as leucine and voriconazole, for which multiple previous selection attempts failed, we designed tailored strategies focused on overcoming individual structural barriers to successful selections. This approach enables us to move beyond standardized protocols into functional group-guided searches, relying on sequences common to receptors for targets and their analogs to serve as anchors in regions of vast oligonucleotide spaces wherein useful reagents are likely to be found.
Databáze: MEDLINE
Nepřihlášeným uživatelům se plný text nezobrazuje