Antimicrobial activity of Desplac® oral gel in the subgingival multispecies biofilm formation.

Autor:	Bueno-Silva B; Dental Research Division, Guarulhos University, Guarulhos, Brazil., Kiausinus KR; Dental Research Division, Guarulhos University, Guarulhos, Brazil., Gonçalves FJDS; Dental Research Division, Guarulhos University, Guarulhos, Brazil., Moreira MVC; Dental Research Division, Guarulhos University, Guarulhos, Brazil., de Oliveira EG; Sysplac Produtos Médicos e Odontológicos LTDA, São Paulo, Brazil., Brugnera Junior A; Education College of the European Master in Oral Laser Application (EMDOLA), University of Liège, Liège, Belgium.; Research Collaborator at the IFSC-University of São Paulo (USP), São Paulo, Brazil., Feres M; Dental Research Division, Guarulhos University, Guarulhos, Brazil., Figueiredo LC; Dental Research Division, Guarulhos University, Guarulhos, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2023 May 16; Vol. 14, pp. 1122051. Date of Electronic Publication: 2023 May 16 (Print Publication: 2023).
DOI:	10.3389/fmicb.2023.1122051
Abstrakt:	Natural products are well-known due to their antimicrobial properties. This study aimed to evaluate the antimicrobial effect of Desplac® product (composed of Aloe Vera, Propolis Extract, Green Tea, Cranberry, and Calendula) on the subgingival biofilm. Two different protocols were used to treat the 33-species biofilms: (A) 2×/day (12/12  h) for 1  min with Desplac® or Noplak Toothpaste (Chlorhexidine + Cetylpyridinium Chloride) or Oral B ProGengiva (stannous Fluoride) or a placebo gel; (B) a 12-h use of the Desplac® product or 0.12% chlorhexidine gel or a placebo gel. After 7 days of biofilm formation, the metabolic activity (MA) and biofilm profile were determined by 2,3,5-triphenyltetrazolium chloride and Checker-board DNA-DNA hybridization, respectively. Statistical analysis used the Kruskal-Wallis test followed by Dunn's post-hoc. In protocol A, all treatments presented reduced MA compared to the placebo ( p  ≤ 0.05). The Desplac®-treated biofilm showed a similar microbial profile to other antimicrobials, although with higher bacterial total counts. In protocol B, MA of Desplac®-treated biofilms was lower than the placebo's MA but higher than chlorhexidine-treated biofilms ( p  ≤ 0.05). Pathogen levels in Desplac®-treated biofilms were lower than in placebo-treated biofilms and elevated compared to the chlorhexidine-treated biofilms ( p  ≤ 0.05). Desplac® inhibited the biofilm development and disrupted the mature subgingival biofilm, highlighting its effect on Tannerella forsythia counts. Competing Interests: EO was the owner of Sysplac Company that produces Desplac product. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Bueno-Silva, Kiausinus, Gonçalves, Moreira, Oliveira, Brugnera Junior, Feres and Figueiredo.)
Databáze:	MEDLINE
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