| Autor: |
Ranjani M; Department of Chemistry, Bharathiar University, Coimbatore 641046, India., Keerthana V; Department of Biochemistry, Bharathiar University, Coimbatore 641046, India., Selvakumar S; Department of Biochemistry, Bharathiar University, Coimbatore 641046, India., Lynch VM; Department of Chemistry, University of Texas, Austin, Texas 78712-1224, United States., Mohankumar A; Department of Zoology, Bharathiar University, Coimbatore 641046, India.; PAK Research Center, University of Ryukyus, Senbaru 1, Nishihara-cho, Okinawa 903-0129, Japan., Palanisamy S; Department of Zoology, Bharathiar University, Coimbatore 641046, India., Kalaivani P; Department of Chemistry, Nirmala College for Women, Coimbatore 641018, India., Prabhakaran R; Department of Chemistry, Bharathiar University, Coimbatore 641046, India. |
| Abstrakt: |
2,3-Dihydro-4-hydroxy-chromene-4( N )-ethyl thiosemicarbazone (probe F4 ) is used as a chemosensor, and it selectively detected Cu 2+ ions among the metal ions by showing fluorescence "TURN ON" behavior. The stoichiometric binding of the probe with Cu 2+ ( CF4 ) was confirmed by Job's plot and mass spectroscopy. Further, CF4 was used as a sensor for the detection of l-amino acids and N-heterocyclic compounds. Among them, CF4 selectively detected l-histidine by showing fluorescence " TURN-OFF " behavior and selectively detected indazole by showing fluorescence " TURN-ON " behavior. These behaviors were further confirmed by in vivo live cell imaging studies by using Caenorhabditis elegans as a model. In vitro cytotoxicity was assayed for probe F4 , complex CF4 , and CF4 with l-histidine and indazole. The IC 50 concentration was used for confocal imaging studies by using the MDA-MB-231 cell line (breast cancer cell line). |
