Empagliflozin Protects against Haloperidol Experimentally-Induced Ovarian Toxicity.

Autor: Abdelzaher WY; Department of Pharmacology, Faculty of Medicine, Minia University, Minia 61511, Egypt., De Waard M; L'institut Du Thorax, INSERM, CNRS, UNIV NANTES, F-44007 Nantes, France.; Smartox Biotechnology, 6 Rue des Platanes, F-38120 Saint-Egrève, France.; LabEx «Ion Channels, Science & Therapeutics», Université de Nice Sophia-Antipolis, F-06560 Valbonne, France., Abdelmonaem AA; Department of Pharmacology, Faculty of Medicine, Minia University, Minia 61511, Egypt., Ali DM; Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Minia University, Minia 61511, Egypt., El-Tahawy NFG; Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Minia 61511, Egypt., Rifaai RA; Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Minia 61511, Egypt., Mohamed HA; Department of Biochemistry, Faculty of Medicine, Minia University, Minia 61511, Egypt., Shaheen K; Department of Obstetrics & Gynecology, Faculty of Medicine, Minia University, Minia 61511, Egypt., Zeen El-Din MA; Department of Obstetrics & Gynecology, Faculty of Medicine, Minia University, Minia 61511, Egypt., Welson NN; Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Beni-Suef University, Beni Suef 62511, Egypt., Tawfeek SE; Anatomy Department, College of Medicine, Jouf University, Sakaka 72388, Saudi Arabia.; Human Anatomy and Embryology Department, Faculty of Medicine, Zagazig University, Sharkia 44519, Egypt., Batiha GE; Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt., Abdel-Aziz AM; Department of Pharmacology, Faculty of Medicine, Minia University, Minia 61511, Egypt.
Jazyk: angličtina
Zdroj: Pharmaceuticals (Basel, Switzerland) [Pharmaceuticals (Basel)] 2023 Jan 23; Vol. 16 (2). Date of Electronic Publication: 2023 Jan 23.
DOI: 10.3390/ph16020168
Abstrakt: The present experiment aimed to identify the potential protective role of empagliflozin (EMPA) on haloperidol (HAL)-induced ovarian damage in female rats because of its anti-inflammatory, antioxidant, and antiapoptotic effects. EMPA was administered in the presence and absence of HAL. Thirty-two adult female albino rats were divided into four groups. Control group, EMPA group: received EMPA (10 mg/kg/day) p.o., HAL group: received HAL (2 mg/kg/day) p.o., HAL + EMPA group: HAL (2 mg/kg/day) combined with EMPA for 28 days. Serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), and anti-mullerian hormone (AMH) levels were measured. Ovarian oxidative stress parameters, besides inflammatory and apoptotic biomarkers, and ovarian Sirtuin-1 (Sirt-1) were evaluated. Ovarian histopathological examination and heat shock protein 70 (Hsp70) immunohistochemical study were performed. HAL significantly increased serum levels of FSH, LH, and ovarian inflammatory, apoptotic, and oxidative stress biomarkers and decreased serum AMH levels and Sirt-1 expression. Histopathological findings of ovarian damage and high Hsp70 immunoexpression were detected. EMPA significantly normalized the distributed hormonal levels, oxidative stress, inflammatory, and apoptotic biomarkers with a prompt improvement in the histopathological picture and a decrease in Hsp70 immunoexpression. Accordingly, EMPA protected against HAL-induced ovarian toxicity by modulating the Sirt-1/Hsp70/TNF-α/caspase-3 signaling pathway.
Competing Interests: The authors declare no conflicts of interest.
Databáze: MEDLINE
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