Caged luciferins enable rapid multicomponent bioluminescence imaging.

Autor: Navarro MX; Department of Chemistry, University of California, Irvine, Irvine, California, USA., Brennan CK; Department of Chemistry, University of California, Irvine, Irvine, California, USA., Love AC; Department of Chemistry, University of California, Irvine, Irvine, California, USA., Prescher JA; Department of Chemistry, University of California, Irvine, Irvine, California, USA.; Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, California, USA.; Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, California, USA.
Jazyk: angličtina
Zdroj: Photochemistry and photobiology [Photochem Photobiol] 2024 Jan-Feb; Vol. 100 (1), pp. 67-74. Date of Electronic Publication: 2023 May 31.
DOI: 10.1111/php.13814
Abstrakt: Bioluminescence is a sensitive technique for imaging biological features over time. Historically, though, the modality has been challenging to employ for multiplexed tracking due to a lack of resolvable luciferase-luciferin pairs. Recent years have seen the development of numerous orthogonal probes for multi-parameter imaging. While successful, generating such tools often requires complex syntheses and lengthy enzyme evolution campaigns. This work showcases an alternative strategy for multiplexed bioluminescence that takes advantage of already-orthogonal caged luciferins and established uncaging enzymes. These probes generate unique bioluminescent signals that can be distinguished via a linear unmixing algorithm. Caged luciferins enabled two- and three-component imaging on the minutes time scale. We further showed that the tools can be used in conjunction with endogenous enzymes for multiplexed studies. Collectively, this approach lowers the barrier to multicomponent bioluminescence imaging and can be readily adopted by the broader community.
(© 2023 The Authors. Photochemistry and Photobiology published by Wiley Periodicals LLC on behalf of American Society for Photobiology.)
Databáze: MEDLINE
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