Sarcosine sensitivity in Escherichia coli is mediated by activation of the glycine cleavage system.

Autor:	Doroshenko VG; Joint-Stock Company 'Ajinomoto-Genetika Research Institute', Moscow, Russia., Slesareva AE; Joint-Stock Company 'Ajinomoto-Genetika Research Institute', Moscow, Russia., Shmonova EA; Joint-Stock Company 'Ajinomoto-Genetika Research Institute', Moscow, Russia., Kivero AD; Joint-Stock Company 'Ajinomoto-Genetika Research Institute', Moscow, Russia.
Jazyk:	angličtina
Zdroj:	Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2023 Jul 24; Vol. 87 (8), pp. 916-924.
DOI:	10.1093/bbb/zbad069
Abstrakt:	Corynebacterium glutamicum AJ1511 and Escherichia coli BW25113 strains were compared in terms of resistance to sarcosine (N-methylglycine). The E. coli strain was more sensitive to sarcosine than C. glutamicum, especially when grown in minimal medium. Growth inhibition of the BW25113 strain in minimal M9 medium containing 0.5 m sarcosine was overcome by the addition of glycine. Inactivation of the glycine cleavage (GCV) system (∆gcvP) as well as the removal of its activator (∆gcvA) in BW25113 cells increased the threshold for sarcosine inhibition up to 0.75 m. Activation of the promoter of the E. coli gcvTHP operon by 0.1-0.4 m sarcosine added to M9 medium was demonstrated in vivo using dasherGFP as the reporter. Sensitivity to sarcosine on glucose minimal medium is suggested to be a characteristic of Gram-negative bacteria with GcvA/GcvR regulation of the GCV system. (© The Author(s) 2023. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.)
Databáze:	MEDLINE
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