Epitope Binning of Monoclonal and Polyclonal Antibodies by Biolayer Interferometry.

Autor:	Nagashima K; Center for Vaccines and Immunology, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.; Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, USA., Mousa JJ; Center for Vaccines and Immunology, College of Veterinary Medicine, University of Georgia, Athens, GA, USA. jarrod.mousa@uga.edu.; Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, USA. jarrod.mousa@uga.edu.; Department of Biochemistry and Molecular Biology, Franklin College of Arts and Sciences, University of Georgia, Athens, GA, USA. jarrod.mousa@uga.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2673, pp. 17-32.
DOI:	10.1007/978-1-0716-3239-0_2
Abstrakt:	Understanding the epitopes of antibodies elicited by infection and vaccination is often useful in immunogen design. In this chapter, we describe biolayer interferometry (BLI)-based methods to evaluate such epitopes and permit simultaneous analysis of antibodies from several sources, including monoclonal antibodies (mAbs) and polyclonal serum antibodies (pAbs). Using previously characterized antibodies with known epitopes as controls, the distribution of epitopes for the influenza hemagglutinin (HA) is shown for isolated human mAbs and pooled serum from HA-immunized mice. This method is versatile, high-throughput, and can be adapted to several antigens. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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