Optimising the yield from bronchoalveolar lavage on human participants in infectious disease immunology research.

Autor: Shaw JA; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa. janeshaw@sun.ac.za.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa. janeshaw@sun.ac.za.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa. janeshaw@sun.ac.za., Meiring M; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa.; South African Tuberculosis Bioinformatics Initiative, Stellenbosch University, Cape Town, South Africa., Allies D; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa., Cruywagen L; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa., Fisher TL; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa., Kasavan K; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa., Roos K; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa., Botha SM; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa., MacDonald C; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa., Hiemstra AM; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa., Simon D; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa., van Rensburg I; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa., Flinn M; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa., Shabangu A; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa., Kuivaniemi H; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa., Tromp G; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa.; South African Tuberculosis Bioinformatics Initiative, Stellenbosch University, Cape Town, South Africa.; Centre for Bioinformatics and Computational Biology, Stellenbosch University, Stellenbosch, South Africa., Malherbe ST; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa., Walzl G; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa., du Plessis N; DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; South African Medical Research Council Centre for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.; Division of Molecular Biology and Human Genetics, Biomedical Research Institute, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 May 31; Vol. 13 (1), pp. 8859. Date of Electronic Publication: 2023 May 31.
DOI: 10.1038/s41598-023-35723-2
Abstrakt: Bronchoalveolar lavage (BAL) is becoming a common procedure for research into infectious disease immunology. Little is known about the clinical factors which influence the main outcomes of the procedure. In research participants who underwent BAL according to guidelines, the BAL volume yield, and cell yield, concentration, viability, pellet colour and differential count were analysed for association with important participant characteristics such as active tuberculosis (TB) disease, TB exposure, HIV infection and recent SARS-CoV-2 infection. In 337 participants, BAL volume and BAL cell count were correlated in those with active TB disease, and current smokers. The right middle lobe yielded the highest volume. BAL cell and volume yields were lower in older participants, who also had more neutrophils. Current smokers yielded lower volumes and higher numbers of all cell types, and usually had a black pellet. Active TB disease was associated with higher cell yields, but this declined at the end of treatment. HIV infection was associated with more bloody pellets, and recent SARS-CoV-2 infection with a higher proportion of lymphocytes. These results allow researchers to optimise their participant and end assay selection for projects involving lung immune cells.
(© 2023. The Author(s).)
Databáze: MEDLINE
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