Antimicrobial susceptibility and molecular characteristics of Mycoplasma bovis isolated from cases of bovine respiratory disease in Australian feedlot cattle.

Autor: Hasoon MF; Australian Center for Antimicrobial Resistance Ecology, School of Animal & Veterinary Sciences, The University of Adelaide, Australia; The Davies Livestock Research Center, School of Animal and Veterinary Sciences, The University of Adelaide, Adelaide, Australia. Electronic address: mauida.alkhallawi@adelaide.edu.au., Jarocki VM; Australian Institute for Microbiology & Infection, University of Technology Sydney, Ultimo, NSW, Australia., Mohammed MH; Australian Center for Antimicrobial Resistance Ecology, School of Animal & Veterinary Sciences, The University of Adelaide, Australia., Djordjevic SP; Australian Institute for Microbiology & Infection, University of Technology Sydney, Ultimo, NSW, Australia., Yip HYE; Australian Center for Antimicrobial Resistance Ecology, School of Animal & Veterinary Sciences, The University of Adelaide, Australia., Carr M; Australian Center for Antimicrobial Resistance Ecology, School of Animal & Veterinary Sciences, The University of Adelaide, Australia; The Davies Livestock Research Center, School of Animal and Veterinary Sciences, The University of Adelaide, Adelaide, Australia., Khabiri A; Australian Center for Antimicrobial Resistance Ecology, School of Animal & Veterinary Sciences, The University of Adelaide, Australia., Azari AA; Department of Microbiology, Gorgan Branch, Islamic Azad University, Gorgan, Iran., Amanollahi R; Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran., Jozani RJ; Australian Center for Antimicrobial Resistance Ecology, School of Animal & Veterinary Sciences, The University of Adelaide, Australia., Carracher B; Warrnambool Veterinary Clinic, Industry, Australia., Mollinger J; Biosecurity Sciences Laboratory, Department of Agriculture and Fisheries, 4108 QLD, Australia., Deutscher AT; Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, 2568 NSW, Australia., Hemmatzadeh F; Australian Center for Antimicrobial Resistance Ecology, School of Animal & Veterinary Sciences, The University of Adelaide, Australia; The Davies Livestock Research Center, School of Animal and Veterinary Sciences, The University of Adelaide, Adelaide, Australia., Trott DJ; Australian Center for Antimicrobial Resistance Ecology, School of Animal & Veterinary Sciences, The University of Adelaide, Australia.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2023 Aug; Vol. 283, pp. 109779. Date of Electronic Publication: 2023 May 23.
DOI: 10.1016/j.vetmic.2023.109779
Abstrakt: To date, antimicrobial susceptibility has not been reported for Australian Mycoplasma bovis isolates. This study determined minimal inhibitory concentrations (MICs) for 12 different antimicrobials against Australian M. bovis isolates and used whole genome sequencing to screen those showing high macrolide MICs for point mutations in target genes. Most lung tissue/swab samples from bovine respiratory disease cases (61/76, 80.3%) tested positive for M. bovis. A set of 50 representative isolates (50/61, 82.0%) that showed adequate growth, was used for MIC testing. Uniformly, low MIC values were confirmed for enrofloxacin (≤ 4 μg/mL), florfenicol (≤ 8 μg/mL), gamithromycin (≤ 2 μg/mL), spectinomycin (≤ 4 μg/mL), tetracycline (≤ 8 μg/mL), tiamulin (≤ 4 μg/mL), and tulathromycin (≤ 0.5 μg/mL). A small proportion (10%) of isolates exhibited high MICs (≥ 32 μg/mL) for tildipirosin, tilmicosin, tylosin, and lincomycin, which were above the epidemiological cut-off values for each antimicrobial (≥ 4 μg/mL). These isolates, originating from three Australian states, underwent whole genome sequencing/multilocus sequencing typing and were compared with the reference strain PG45 to investigate mutations that might be linked with the high macrolide/lincosamide MICs. All five belonged to ST52 and two macrolide associated mutations were identified within the 23 S rRNA gene (A2058G in two sequenced isolates and G748A in all sequenced isolates). Four additional 23 S rRNA gene mutations did not appear to be linked to macrolide resistance. Whilst the majority of Australian M. bovis isolates appear susceptible to the tested antimicrobials, emerging macrolide resistance was detected in three Australian states and requires continued monitoring.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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