Molecularly imprinted electrochemical sensor for the selective and sensitive determination of octreotide in cancer patient plasma sample.

Autor: Ozkan E; Ankara Medipol University, Faculty of Pharmacy, Analytical Chemistry Department, Ankara, 06790, Turkiye. Electronic address: ecedonmezoglu@gmail.com., Ozcelikay G; Ankara University, Faculty of Pharmacy, Analytical Chemistry Department, Ankara, 06560, Turkiye., Gök Topak ED; Hacettepe University, Faculty of Pharmacy, Analytical Chemistry Department, Ankara, 06100, Turkiye; Lokman Hekim University, Faculty of Pharmacy, Analytical Chemistry Department, Ankara, 06510, Turkiye., Nemutlu E; Hacettepe University, Faculty of Pharmacy, Analytical Chemistry Department, Ankara, 06100, Turkiye., Ozkan SA; Ankara University, Faculty of Pharmacy, Analytical Chemistry Department, Ankara, 06560, Turkiye. Electronic address: ozkan@pharmacy.ankara.edu.tr., Dizdar Ö; Hacettepe University, Cancer Institute, Medical Oncology Department, Ankara, 06100, Turkiye., Aksoy S; Hacettepe University, Cancer Institute, Medical Oncology Department, Ankara, 06100, Turkiye., Kır S; Hacettepe University, Faculty of Pharmacy, Analytical Chemistry Department, Ankara, 06100, Turkiye.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2023 Oct 01; Vol. 263, pp. 124679. Date of Electronic Publication: 2023 May 19.
DOI: 10.1016/j.talanta.2023.124679
Abstrakt: In this study, a molecularly imprinted polymer film (P (ANI)@MIP) on the electrode surface was fabricated using aniline as a functional monomer and octreotide (OC) as a template molecule. The developed P (ANI)@MIP was electrochemically electropolymerized on a glassy carbon electrode (GCE) surface. Each step of MIP production was evaluated by viewing the [Fe (CN) 6 ] 3-/4- signal obtained using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The P (ANI)@MIP film layer was studied with a scanning electron microscope (SEM), Raman, and contact angle measurements. The parameters consisting of monomer, template ratio, cycle number, removal solution, removal time, and rebinding time were optimized to obtain the best electrochemical sensor. The developed method was validated in line with ICH guidelines. The linear range, LOD, and LOQ were found as 10-80 fM, 0.801 fM, and 2.670 fM, respectively. The selectivity of the method was tested with the response of somatostatin and lanreotide from the same growth hormone family by comparing the OC response. The developed P (ANI)@MIP/GCE sensor is the first reported method for electrochemical analysis of OC. The P (ANI)@MIP/GCE sensor exhibited high sensitivity and selectivity for OC. The novel MIP sensor was used to determine OC in cancer patient plasma samples. The concentration of OC in cancer patients varied between 8.98 ng/mL and 10.10 ng/mL.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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