Accelerated Hemocompatibility Testing of Rotary Blood Pumps.

Autor: McNamee AP; From the Biorheology Research Laboratory, Menzies Health Institute Queensland, Griffith University, Gold Coast, Queensland, Australia., Griffith TA, Smith AG, Kuck L, Simmonds MJ
Jazyk: angličtina
Zdroj: ASAIO journal (American Society for Artificial Internal Organs : 1992) [ASAIO J] 2023 Oct 01; Vol. 69 (10), pp. 918-923. Date of Electronic Publication: 2023 Sep 27.
DOI: 10.1097/MAT.0000000000001995
Abstrakt: Ex vivo hemocompatibility testing is a vital element of preclinical assessment for blood-contacting medical devices. Current approaches are resource intensive; thus, we investigated the feasibility of accelerating hemocompatibility testing by standardizing the number of pump exposures in loops of various sizes. Three identical blood loops were constructed, each with a custom-molded reservoir able to facilitate large-volume expansion. Using the HVAD rotary blood pump operating at 5 L·min -1 and 100 mmHg, three test volumes (80, 160, and 320 ml) were circulated for 4000 pump exposures. Blood sampling was performed at individualized intervals every one-sixth of total duration for the assessment of hemolysis and von Willebrand Factor (vWF) degradation. While steady increases in hemolysis (~24 mg·dl -1 ) were identified in all tests at completion, loop volume was not a primary discriminator. The normalized index of hemolysis did not vary significantly between loops (4.2-4.9 mg·100 L -1 ). vWF degradation progressively occurred with duration of testing to a similar extent under all conditions. These data support an accelerated approach to preclinical assessment of ex vivo blood damage. Adopting this approach enables: enhanced efficiency for rapid prototyping; reduced ex vivo blood aging, and; greater utility of blood, which is presently limited if 450 ml loops are desired.
Competing Interests: Disclosure: The authors have no conflicts of interest to report.
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Databáze: MEDLINE