Biting midges (Diptera: Ceratopogonidae) as putative vectors of zoonotic Onchocerca lupi (Nematoda: Onchocercidae) in northern Arizona and New Mexico, southwestern United States.

Autor:	Roe CC; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, United States.; School of Informatics, Computing, and Cyber Systems, Northern Arizona University, Flagstaff, AZ, United States., Holiday O; Navajo Nation Veterinary Management Program, Window Rock, AZ, United States., Upshaw-Bia K; Navajo Nation Veterinary Management Program, Window Rock, AZ, United States., Benally G; Navajo Nation Veterinary Management Program, Window Rock, AZ, United States., Williamson CHD; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, United States., Urbanz J; Eye Care for Animals, Scottsdale, AZ, United States., Verocai GG; Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, United States., Ridenour CL; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, United States.; School of Informatics, Computing, and Cyber Systems, Northern Arizona University, Flagstaff, AZ, United States.; Pathogen and Microbiome Division, Translational Genomics Research Institute, Flagstaff, AZ, United States., Nottingham R; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, United States., Ford MA; U.S. Geological Survey, Southwest Biological Science Center, Flagstaff, AZ, United States., Lake DP; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, United States., Kennedy TA; U.S. Geological Survey, Southwest Biological Science Center, Flagstaff, AZ, United States., Hepp CM; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, United States.; School of Informatics, Computing, and Cyber Systems, Northern Arizona University, Flagstaff, AZ, United States.; Pathogen and Microbiome Division, Translational Genomics Research Institute, Flagstaff, AZ, United States., Sahl JW; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2023 May 15; Vol. 10, pp. 1167070. Date of Electronic Publication: 2023 May 15 (Print Publication: 2023).
DOI:	10.3389/fvets.2023.1167070
Abstrakt:	Onchocerca lupi (Rodonaja, 1967) is an understudied, vector-borne, filarioid nematode that causes ocular onchocercosis in dogs, cats, coyotes, wolves, and is also capable of infecting humans. Onchocercosis in dogs has been reported with increasing incidence worldwide. However, despite the growing number of reports describing canine O. lupi cases as well as zoonotic infections globally, the disease prevalence in endemic areas and vector species of this parasite remains largely unknown. Here, our study aimed to identify the occurrence of O. lupi infected dogs in northern Arizona, New Mexico, and Utah, United States and identify the vector of this nematode. A total of 532 skin samples from randomly selected companion animals with known geographic locations within the Navajo Reservation were collected and molecularly surveyed by PCR for the presence of O. lupi DNA (September 2019-June 2022) using previously published nematode primers (COI) and DNA sequencing. O. lupi DNA was detected in 50 (9.4%) sampled animals throughout the reservation. Using positive animal samples to target geographic locations, pointed hematophagous insect trapping was performed to identify potential O. lupi vectors. Out of 1,922 insects screened, 38 individual insects and 19 insect pools tested positive for the presence of O. lupi, all of which belong to the Diptera family. This increased surveillance of definitive host and biological vector/intermediate host is the first large scale prevalence study of O. lupi in companion animals in an endemic area of the United States, and identified an overall prevalence of 9.4% in companion animals as well as multiple likely biological vector and putative vector species in the southwestern United States. Furthermore, the identification of these putative vectors in close proximity to human populations coupled with multiple, local zoonotic cases highlight the One Health importance of O. lupi . Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Roe, Holiday, Upshaw-Bia, Benally, Williamson, Urbanz, Verocai, Ridenour, Nottingham, Ford, Lake, Kennedy, Hepp and Sahl.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu