Alterations of bovine nucleus pulposus cells with aging.

Autor: Molinos M; i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal.; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal., Fiordalisi MF; i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal.; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal., Caldeira J; i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal., Almeida CR; i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal.; iBiMED - Institute of Biomedicine, Department of Medical Sciences, University of Aveiro, Aveiro, Portugal., Barbosa MA; i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal.; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal., Gonçalves RM; i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal.; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal.
Jazyk: angličtina
Zdroj: Aging cell [Aging Cell] 2023 Aug; Vol. 22 (8), pp. e13873. Date of Electronic Publication: 2023 May 30.
DOI: 10.1111/acel.13873
Abstrakt: Aging is one of the major etiological factors driving intervertebral disc (IVD) degeneration, the main cause of low back pain. The nucleus pulposus (NP) includes a heterogeneous cell population, which is still poorly characterized. Here, we aimed to uncover main alterations in NP cells with aging. For that, bovine coccygeal discs from young (12 months) and old (10-16 years old) animals were dissected and primary NP cells were isolated. Gene expression and proteomics of fresh NP cells were performed. NP cells were labelled with propidium iodide and analysed by flow cytometry for the expression of CD29, CD44, CD45, CD146, GD2, Tie2, CD34 and Stro-1. Morphological cell features were also dissected by imaging flow cytometry. Elder NP cells (up-regulated bIL-6 and bMMP1 gene expression) presented lower percentages of CD29+, CD44+, CD45+ and Tie2+ cells compared with young NP cells (upregulated bIL-8, bCOL2A1 and bACAN gene expression), while GD2, CD146, Stro-1 and CD34 expression were maintained with age. NP cellulome showed an upregulation of proteins related to endoplasmic reticulum (ER) and melanosome independently of age, whereas proteins upregulated in elder NP cells were also associated with glycosylation and disulfide bonds. Flow cytometry analysis of NP cells disclosed the existence of 4 subpopulations with distinct auto-fluorescence and size with different dynamics along aging. Regarding cell morphology, aging increases NP cell area, diameter and vesicles. These results contribute to a better understanding of NP cells aging and highlighting potential anti-aging targets that can help to mitigate age-related disc disease.
(© 2023 The Authors. Aging Cell published by Anatomical Society and John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje