Hydrogel-in-hydrogel live bioprinting for guidance and control of organoids and organotypic cultures.

Autor: Urciuolo A; Dept. of Molecular Medicine, University of Padova, Padova, Italy. anna.urciuolo@unipd.it.; Istituto di Ricerca Pediatrica, Città della Speranza, Padova, Italy. anna.urciuolo@unipd.it., Giobbe GG; GOSICH Zayed Centre for Research into Rare Disease in Children, University College London, London, UK., Dong Y; Shanghai Institute for Advanced Immunochemical Studies (SIAIS), ShanghaiTech University, Shanghai, China., Michielin F; GOSICH Zayed Centre for Research into Rare Disease in Children, University College London, London, UK., Brandolino L; Dept. of Industrial Engineering, University of Padova, Padova, Italy.; Veneto Institute of Molecular Medicine, Padova, Italy., Magnussen M; GOSICH Zayed Centre for Research into Rare Disease in Children, University College London, London, UK., Gagliano O; Dept. of Industrial Engineering, University of Padova, Padova, Italy.; Veneto Institute of Molecular Medicine, Padova, Italy., Selmin G; GOSICH Zayed Centre for Research into Rare Disease in Children, University College London, London, UK., Scattolini V; Istituto di Ricerca Pediatrica, Città della Speranza, Padova, Italy., Raffa P; Istituto di Ricerca Pediatrica, Città della Speranza, Padova, Italy., Caccin P; Dept. of Biomedical Science, University of Padova, Padova, Italy., Shibuya S; GOSICH Zayed Centre for Research into Rare Disease in Children, University College London, London, UK., Scaglioni D; GOSICH Zayed Centre for Research into Rare Disease in Children, University College London, London, UK., Wang X; Shanghai Institute for Advanced Immunochemical Studies (SIAIS), ShanghaiTech University, Shanghai, China., Qu J; Shanghai Institute for Advanced Immunochemical Studies (SIAIS), ShanghaiTech University, Shanghai, China., Nikolic M; GOSICH Zayed Centre for Research into Rare Disease in Children, University College London, London, UK., Montagner M; Dept. of Molecular Medicine, University of Padova, Padova, Italy., Galea GL; GOSICH Zayed Centre for Research into Rare Disease in Children, University College London, London, UK., Clevers H; Hubrecht Institute, KNAW and University Medical Center, Utrecht, The Netherlands.; Pharma Research and Early Development (pRED) of Roche, Basel, Switzerland., Giomo M; Dept. of Industrial Engineering, University of Padova, Padova, Italy., De Coppi P; GOSICH Zayed Centre for Research into Rare Disease in Children, University College London, London, UK.; Dept. of Specialist Neonatal and Paediatric Surgery, Great Ormond Street Hospital, London, UK., Elvassore N; GOSICH Zayed Centre for Research into Rare Disease in Children, University College London, London, UK. nicola.elvassore@unipd.it.; Dept. of Industrial Engineering, University of Padova, Padova, Italy. nicola.elvassore@unipd.it.; Veneto Institute of Molecular Medicine, Padova, Italy. nicola.elvassore@unipd.it.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 May 30; Vol. 14 (1), pp. 3128. Date of Electronic Publication: 2023 May 30.
DOI: 10.1038/s41467-023-37953-4
Abstrakt: Three-dimensional hydrogel-based organ-like cultures can be applied to study development, regeneration, and disease in vitro. However, the control of engineered hydrogel composition, mechanical properties and geometrical constraints tends to be restricted to the initial time of fabrication. Modulation of hydrogel characteristics over time and according to culture evolution is often not possible. Here, we overcome these limitations by developing a hydrogel-in-hydrogel live bioprinting approach that enables the dynamic fabrication of instructive hydrogel elements within pre-existing hydrogel-based organ-like cultures. This can be achieved by crosslinking photosensitive hydrogels via two-photon absorption at any time during culture. We show that instructive hydrogels guide neural axon directionality in growing organotypic spinal cords, and that hydrogel geometry and mechanical properties control differential cell migration in developing cancer organoids. Finally, we show that hydrogel constraints promote cell polarity in liver organoids, guide small intestinal organoid morphogenesis and control lung tip bifurcation according to the hydrogel composition and shape.
(© 2023. The Author(s).)
Databáze: MEDLINE
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